| Isolation of salmonellas by immunomagnetic separation. | |
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MedLine Citation:
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PMID: 1556036 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Magnetisable particles, coated with anti-salmonella serum, were used to isolate Salmonella livingstone from pure cultures, mixed cultures and food samples. Beads (10(7] were generally incubated with 10(4) Salm. livingstone cells/ml for 60 min at room temperature. The incubation and washing medium (0.01 mol/l phosphate-buffered saline; PBS) contained 0.1% bovine serum albumin (BSA) and 0.1% Tween 20, respectively. This method gave a recovery for Salm. livingstone of 51.0 +/- 7.8%. However, other micro-organisms such as Aeromonas hydrophila interfered with this test because of non-specific reactions (recovery 50.9 +/- 12.7%). These non-specific reactions could be decreased by using 4% skim milk instead of 0.1% BSA in the incubation medium. The ratio of the recovery of Salm. livingstone relative to the recovery of Aer. hydrophila changed from 0.9 when PBS with 0.1% BSA was used, to 13.4 when PBS with 4% skim milk was used. Immunomagnetic separation of Salmonella spp. from food samples offers good prospects for concentrating salmonella cells from heterogeneous bacterial suspensions, such as enrichment broths. |
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Authors:
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A E Vermunt; A A Franken; R R Beumer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of applied bacteriology Volume: 72 ISSN: 0021-8847 ISO Abbreviation: J. Appl. Bacteriol. Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-05-07 Completed Date: 1992-05-07 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 7503050 Medline TA: J Appl Bacteriol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 112-8 Citation Subset: IM |
Affiliation:
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State Institute for Quality Control of Agricultural Products (RIKILT-DLO), Wageningen, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Culture Media Fluorescent Antibody Technique Food Microbiology* Magnetics Meat Microspheres Salmonella / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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