Document Detail


Isolation of salmonellas by immunomagnetic separation.
MedLine Citation:
PMID:  1556036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Magnetisable particles, coated with anti-salmonella serum, were used to isolate Salmonella livingstone from pure cultures, mixed cultures and food samples. Beads (10(7] were generally incubated with 10(4) Salm. livingstone cells/ml for 60 min at room temperature. The incubation and washing medium (0.01 mol/l phosphate-buffered saline; PBS) contained 0.1% bovine serum albumin (BSA) and 0.1% Tween 20, respectively. This method gave a recovery for Salm. livingstone of 51.0 +/- 7.8%. However, other micro-organisms such as Aeromonas hydrophila interfered with this test because of non-specific reactions (recovery 50.9 +/- 12.7%). These non-specific reactions could be decreased by using 4% skim milk instead of 0.1% BSA in the incubation medium. The ratio of the recovery of Salm. livingstone relative to the recovery of Aer. hydrophila changed from 0.9 when PBS with 0.1% BSA was used, to 13.4 when PBS with 4% skim milk was used. Immunomagnetic separation of Salmonella spp. from food samples offers good prospects for concentrating salmonella cells from heterogeneous bacterial suspensions, such as enrichment broths.
Authors:
A E Vermunt; A A Franken; R R Beumer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of applied bacteriology     Volume:  72     ISSN:  0021-8847     ISO Abbreviation:  J. Appl. Bacteriol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-05-07     Completed Date:  1992-05-07     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  7503050     Medline TA:  J Appl Bacteriol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  112-8     Citation Subset:  IM    
Affiliation:
State Institute for Quality Control of Agricultural Products (RIKILT-DLO), Wageningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Culture Media
Fluorescent Antibody Technique
Food Microbiology*
Magnetics
Meat
Microspheres
Salmonella / isolation & purification*
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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