Document Detail


Isolation and purification of an unspecific component from human serum albumin, typical for pathology
MedLine Citation:
PMID:  7281554     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Albumin from blood serum of healthy persons and from patients with various pathologies and different severity of diseases was characterized using isoelectric focusing in borate-polyol system. In all the pathologies studied a new component occurred, which had an isoelectric point at pH 5.5 and which was not found in fresh albumin preparations isolated from healthy persons. This fraction was isolated and purified. Its isoelectric point was near pH 4.5-4.6 in isoelectrofocusing with ampholines. The divergences were due to complex formation of albumin with ampholines. The modified protein corresponded immunochemically to the human blood serum albumin, did not contain polymers, had a decreased amount of alpha-helix structures as shown by dispersion of optic rotation and its molecular mass was similar to the mass of native albumin.
Authors:
G V Troitskiĭ; M Akhmed; G Iu Azhitskiĭ; S N Bagdasar'ian; V A Gaevskaia
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Voprosy medit͡sinskoĭ khimii     Volume:  27     ISSN:  0042-8809     ISO Abbreviation:  Vopr. Med. Khim.     Publication Date:    1981 Mar-Apr
Date Detail:
Created Date:  1981-11-18     Completed Date:  1981-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0416601     Medline TA:  Vopr Med Khim     Country:  USSR    
Other Details:
Languages:  rus     Pagination:  166-72     Citation Subset:  IM    
Vernacular Title:
Vydelenie i ochistka nespetsificheskogo komponenta syvorotochnogo al bumina cheloveka, kharakternogo dlia patologii.
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MeSH Terms
Descriptor/Qualifier:
Humans
Isoelectric Focusing / methods
Laboratory Techniques and Procedures*
Protein Conformation
Reference Values
Serum Albumin / isolation & purification*
Chemical
Reg. No./Substance:
0/Serum Albumin

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