Document Detail


Isolation and purification of primary rodent astrocytes.
MedLine Citation:
PMID:  18428469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Astrocytes are a major cell type in the mammalian central nervous system (CNS). The ability to obtain virtually pure populations of these cells makes it possible to study their function as isolated cells or in mixed populations where they support the growth and survival of surrounding neurons. Unlike other mature CNS cells, mature astrocytes maintain the lifelong ability to reenter the cell cycle. The first isolation procedure described in this unit takes advantage of the proliferative ability of these cells, as does the second, except that no antibody or complement treatment is required. A procedure for detecting glial fibrillary acidic protein (GFAP), which is present in most astrocytes in vivo and virtually all astrocytes in vitro and is a useful marker for assessing the purity of cultures, is also presented.
Authors:
D E Weinstein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]     Volume:  Chapter 3     ISSN:  1934-8576     ISO Abbreviation:  Curr Protoc Neurosci     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-06-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706581     Medline TA:  Curr Protoc Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 3.5     Citation Subset:  IM    
Affiliation:
Albert Einstein College of Medicine, Bronx, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Astrocytes / chemistry,  cytology*,  physiology
Cell Culture Techniques / methods*
Cell Division / physiology
Cell Separation / methods*
Cells, Cultured
Glial Fibrillary Acidic Protein / analysis
Mice
Optic Nerve / chemistry,  cytology
Stem Cells / chemistry,  cytology,  physiology
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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