Document Detail


Isolation, purification, and partial characterization of a lipopolysaccharide from Haemophilus pleuropneumoniae.
MedLine Citation:
PMID:  3943899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipopolysaccharide (LPS) from Haemophilus pleuropneumoniae 1536, serotype 2, was isolated and purified by a procedure designed to be equally satisfactory for both smooth- and rough-type LPS. The LPS yield was 53%. Analysis of the preparations revealed that protein, nucleic acid, and cellular phospholipid contamination was negligible (less than 0.1%). Analysis of the sugar content of the LPS by gas-liquid chromatography and colorimetric analysis revealed the presence of rhamnose, mannose, galactose, glucose, heptose, glucosamine, galactosamine, and 3-deoxy-D-manno-2-octulosonic acid. The heptose and glucose contents appeared to be unusually high. The fatty acids of the LPS consisted of a mixture of C14:0 and C16:0 in a ratio of about 4.5:1 (50% of the total) and 3-hydroxy C14:0. When used as a preparatory dose for the dermal Shwartzman reaction, as little as 10 micrograms of the LPS injected intradermally in rabbits produced reddening and swelling. After intravenous injection of a 100-micrograms LPS provoking dose, necrosis was observed at all intradermal injection sites. Limulus amebocyte lysate gelation was observed with an LPS concentration as low as 0.5 ng/ml. A typical biphasic fever response was noted in rabbits injected with as little as 0.25 ng of LPS per kg of body weight.
Authors:
J R Maudsley; S Kadis; W R Mayberry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  51     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-03-07     Completed Date:  1986-03-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  501-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Fatty Acids / analysis
Haemophilus / analysis*
Lipopolysaccharides / analysis,  isolation & purification*,  toxicity
Phospholipids / analysis
Rabbits
Sugar Acids / analysis
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lipopolysaccharides; 0/Phospholipids; 0/Sugar Acids; 1069-03-0/2-keto-3-deoxyoctonate
Comments/Corrections

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