Document Detail


Isolation and purification of an early pregnancy factor-like molecule from culture supernatants obtained from lymphocytes of pregnant women.
MedLine Citation:
PMID:  9547687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Our purpose was to determine whether lymphocytes synthesize proteins during pregnancy, to observe whether one of the proteins synthesized has early pregnancy factor (EPF)-like activity and to isolate and purify this molecule from culture supernatants obtained from stimulated lymphocytes of pregnant women.
METHODS: Lymphocyte proliferation assay and 35S-methionine labeling were done to study de novo synthesis of proteins followed by autoradiography to confirm synthesis of proteins. The rosette inhibition assay was used for detection of the EPF-like molecule. Gel filtration on Sephadex G-100 and RPHPLC were used for purification of the EPF-like molecule.
RESULTS: The rate of incorporation of 35S-methionine was significantly higher in the lymphocytes of pregnant women compared to those of the control, and autoradiography confirmed the synthesis of proteins during pregnancy. There is a total protein enhancement trend observed during the first trimester that declines toward term. The EPF-like molecule is observed to be synthesized during all the trimesters of pregnancy. This molecule, when purified, showed a single homogeneous biologically active peak.
CONCLUSIONS: The results indicated that there is an enhancement of existing protein or synthesis of new proteins during pregnancy. The EPF-like molecule is one of the many proteins synthesized and secreted by lymphocytes during pregnancy that, when purified, is biologically active.
Authors:
C Aranha; U Natraj; K S Iyer; S Shahani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  15     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-06-11     Completed Date:  1998-06-11     Revised Date:  2013-03-26    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-24     Citation Subset:  IM    
Affiliation:
Institute for Research in Reproduction (ICMR), Parel, Mumbai, India.
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MeSH Terms
Descriptor/Qualifier:
Autoradiography
Chaperonin 10
Chromatography, Gel
Chromatography, High Pressure Liquid
Concanavalin A
Electrophoresis, Polyacrylamide Gel
Female
Humans
Isotope Labeling
Lymphocyte Activation
Lymphocytes / immunology,  metabolism*
Male
Peptides / isolation & purification*,  secretion
Pregnancy / immunology*
Pregnancy Proteins*
Pregnancy Trimesters / immunology
Rosette Formation
Suppressor Factors, Immunologic / biosynthesis,  isolation & purification*
Chemical
Reg. No./Substance:
0/Chaperonin 10; 0/Peptides; 0/Pregnancy Proteins; 0/Suppressor Factors, Immunologic; 0/early pregnancy factor; 11028-71-0/Concanavalin A
Comments/Corrections

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