| Isolation and purification of an early pregnancy factor-like molecule from culture supernatants obtained from lymphocytes of pregnant women. | |
| | |
MedLine Citation:
|
PMID: 9547687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: Our purpose was to determine whether lymphocytes synthesize proteins during pregnancy, to observe whether one of the proteins synthesized has early pregnancy factor (EPF)-like activity and to isolate and purify this molecule from culture supernatants obtained from stimulated lymphocytes of pregnant women. METHODS: Lymphocyte proliferation assay and 35S-methionine labeling were done to study de novo synthesis of proteins followed by autoradiography to confirm synthesis of proteins. The rosette inhibition assay was used for detection of the EPF-like molecule. Gel filtration on Sephadex G-100 and RPHPLC were used for purification of the EPF-like molecule. RESULTS: The rate of incorporation of 35S-methionine was significantly higher in the lymphocytes of pregnant women compared to those of the control, and autoradiography confirmed the synthesis of proteins during pregnancy. There is a total protein enhancement trend observed during the first trimester that declines toward term. The EPF-like molecule is observed to be synthesized during all the trimesters of pregnancy. This molecule, when purified, showed a single homogeneous biologically active peak. CONCLUSIONS: The results indicated that there is an enhancement of existing protein or synthesis of new proteins during pregnancy. The EPF-like molecule is one of the many proteins synthesized and secreted by lymphocytes during pregnancy that, when purified, is biologically active. |
| | |
Authors:
|
C Aranha; U Natraj; K S Iyer; S Shahani |
Related Documents
:
|
12034307 - Micronuclei and sister chromatid exchanges induced by capsaicin in human lymphocytes. 19907427 - Maternal obesity: the interplay between inflammation, mother and fetus. 19866377 - Toll-like receptors in pregnancy disorders and placental dysfunction. 2030857 - Cellular immunity in preeclampsia: alterations in t-lymphocyte subpopulations during ea... 438847 - Alcohol embryo- and fetopathy. neuropathology of 3 children and 3 fetuses. 15193867 - Placental protein 13 (pp-13): effects on cultured trophoblasts, and its detection in hu... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of assisted reproduction and genetics Volume: 15 ISSN: 1058-0468 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 1998 Mar |
Date Detail:
|
Created Date: 1998-06-11 Completed Date: 1998-06-11 Revised Date: 2013-03-26 |
Medline Journal Info:
|
Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 117-24 Citation Subset: IM |
Affiliation:
|
Institute for Research in Reproduction (ICMR), Parel, Mumbai, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Autoradiography Chaperonin 10 Chromatography, Gel Chromatography, High Pressure Liquid Concanavalin A Electrophoresis, Polyacrylamide Gel Female Humans Isotope Labeling Lymphocyte Activation Lymphocytes / immunology, metabolism* Male Peptides / isolation & purification*, secretion Pregnancy / immunology* Pregnancy Proteins* Pregnancy Trimesters / immunology Rosette Formation Suppressor Factors, Immunologic / biosynthesis, isolation & purification* |
| Chemical | |
Reg. No./Substance:
|
0/Chaperonin 10; 0/Peptides; 0/Pregnancy Proteins; 0/Suppressor Factors, Immunologic; 0/early pregnancy factor; 11028-71-0/Concanavalin A |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Low multiple pregnancy rates and reduced frequency of cancellation after ovulation induction with go...
Next Document: Performing testicular or epididymal sperm retrieval prior to the injection of hCG.