Document Detail

Isolation, purification and amino acid sequence of a tripeptide from bovine pineal tissue displaying antigonadotropic properties.
MedLine Citation:
PMID:  7357038     Owner:  NLM     Status:  MEDLINE    
Studies were performed to isolate peptides displaying antigonadotropic properties from bovine pineal tissue. Inhibition of compensatory ovarian hypertrophy in adult mice was used as an index of activity to guide the purification of a bovine pineal extract in order to isolate these antigonadal peptides. Defatted bovine pineal glands were extracted with acetic acid and further purified by cation-exchange chromatography, gel filtration and paper electrophoresis. The electrophoretogram revealed sixteen ninhydrin spots, of which four were antigonadotropic. One of these fractions was subjected to paper chromatography which yielded two antigonadal fractions. Amino acid analysis of each of these fractions indicated that one was in pure form and the sequence was found to be threonylserinyllysine. The other fraction was heterogeneous, but contained no lysine. Analysis of the amino acid content of the other antigonadal fractions obtained after electrophoresis indicated the possibility that other peptides were present, but did not suggest the presence of arginine vasotocin.
R J Orts; T H Liao; J L Sartin; B C Bruot
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  628     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1980 Mar 
Date Detail:
Created Date:  1980-05-14     Completed Date:  1980-05-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  201-8     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Chromatography, Gel
Gonadotropins / antagonists & inhibitors*
Hormone Antagonists / isolation & purification*,  pharmacology
Hypertrophy / prevention & control
Oligopeptides / isolation & purification*,  pharmacology
Ovary / pathology
Pineal Gland / analysis*
Reg. No./Substance:
0/Gonadotropins; 0/Hormone Antagonists; 0/Oligopeptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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