| Isolation, purification and amino acid sequence of a tripeptide from bovine pineal tissue displaying antigonadotropic properties. | |
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MedLine Citation:
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PMID: 7357038 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies were performed to isolate peptides displaying antigonadotropic properties from bovine pineal tissue. Inhibition of compensatory ovarian hypertrophy in adult mice was used as an index of activity to guide the purification of a bovine pineal extract in order to isolate these antigonadal peptides. Defatted bovine pineal glands were extracted with acetic acid and further purified by cation-exchange chromatography, gel filtration and paper electrophoresis. The electrophoretogram revealed sixteen ninhydrin spots, of which four were antigonadotropic. One of these fractions was subjected to paper chromatography which yielded two antigonadal fractions. Amino acid analysis of each of these fractions indicated that one was in pure form and the sequence was found to be threonylserinyllysine. The other fraction was heterogeneous, but contained no lysine. Analysis of the amino acid content of the other antigonadal fractions obtained after electrophoresis indicated the possibility that other peptides were present, but did not suggest the presence of arginine vasotocin. |
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Authors:
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R J Orts; T H Liao; J L Sartin; B C Bruot |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 628 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1980 Mar |
Date Detail:
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Created Date: 1980-05-14 Completed Date: 1980-05-14 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 201-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cattle Chromatography, Gel Female Gonadotropins / antagonists & inhibitors* Hormone Antagonists / isolation & purification*, pharmacology Hypertrophy / prevention & control Oligopeptides / isolation & purification*, pharmacology Ovary / pathology Pineal Gland / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Gonadotropins; 0/Hormone Antagonists; 0/Oligopeptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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