Document Detail


Isolation and purification of Aeromonas sobria cytotonic enterotoxin and beta-haemolysin.
MedLine Citation:
PMID:  8384263     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aeromonas sp., grown in tryptone soya broth supplemented with yeast extract, 0.6%, pH 7.5, and incubated with agitation at 100 oscillations/min for 15 h at 37 degrees C produced optimal amounts of beta-haemolysin and cytotonic enterotoxin. More prolonged incubation resulted in the loss of enterotoxic activity and anion exchange chromatographic analysis indicated the presence of a moiety capable of breaking down the toxin. Anion exchange fast protein liquid chromatography resulted in a single peak of haemolytic activity and two peaks with enterotoxic activity. The cytotonic enterotoxin was purified from the fraction most active in the infant mouse assay; the second peak, which did not cross-react immunologically, may represent a second cytotonic enterotoxin. Neither peak was observed in the chromatographic fractions of filtrates from strains devoid of activity in the infant mouse assay. Purified enterotoxin, estimated to have a mol. wt of 15 kDa by SDS-PAGE, caused fluid accumulation in the infant mouse assay, was non-haemolytic to rabbit erythrocytes, caused an increase in cAMP activity in tissue culture cells and did not cross-react immunologically with components of cholera toxin or the whole toxin. Purified beta-haemolysin had an estimated mol. wt of 55 kDa, lysed rabbit erythrocytes and did not cause fluid accumulation in the infant mouse test.
Authors:
P J Gosling; P C Turnbull; N F Lightfoot; J V Pether; R J Lewis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  38     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-04-16     Completed Date:  1993-04-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  227-34     Citation Subset:  IM    
Affiliation:
Public Health Laboratory, Musgrove Park Hospital, Taunton, Somerset.
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MeSH Terms
Descriptor/Qualifier:
Aeromonas / metabolism,  pathogenicity*
Animals
Bacterial Toxins / biosynthesis,  chemistry,  immunology,  isolation & purification*
Cells, Cultured
Chromatography, Gel
Chromatography, Ion Exchange
Cross Reactions
Cyclic AMP / analysis,  biosynthesis
Electrophoresis, Polyacrylamide Gel
Enterotoxins / biosynthesis,  chemistry,  immunology,  isolation & purification*
G(M1) Ganglioside / metabolism
Hemolysin Proteins / biosynthesis,  chemistry,  immunology,  isolation & purification*
Humans
Mice
Molecular Weight
Time Factors
Virulence / physiology
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Enterotoxins; 0/Hemolysin Proteins; 37758-47-7/G(M1) Ganglioside; 60-92-4/Cyclic AMP

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