| Isolation and purification of Aeromonas sobria cytotonic enterotoxin and beta-haemolysin. | |
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MedLine Citation:
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PMID: 8384263 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aeromonas sp., grown in tryptone soya broth supplemented with yeast extract, 0.6%, pH 7.5, and incubated with agitation at 100 oscillations/min for 15 h at 37 degrees C produced optimal amounts of beta-haemolysin and cytotonic enterotoxin. More prolonged incubation resulted in the loss of enterotoxic activity and anion exchange chromatographic analysis indicated the presence of a moiety capable of breaking down the toxin. Anion exchange fast protein liquid chromatography resulted in a single peak of haemolytic activity and two peaks with enterotoxic activity. The cytotonic enterotoxin was purified from the fraction most active in the infant mouse assay; the second peak, which did not cross-react immunologically, may represent a second cytotonic enterotoxin. Neither peak was observed in the chromatographic fractions of filtrates from strains devoid of activity in the infant mouse assay. Purified enterotoxin, estimated to have a mol. wt of 15 kDa by SDS-PAGE, caused fluid accumulation in the infant mouse assay, was non-haemolytic to rabbit erythrocytes, caused an increase in cAMP activity in tissue culture cells and did not cross-react immunologically with components of cholera toxin or the whole toxin. Purified beta-haemolysin had an estimated mol. wt of 55 kDa, lysed rabbit erythrocytes and did not cause fluid accumulation in the infant mouse test. |
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Authors:
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P J Gosling; P C Turnbull; N F Lightfoot; J V Pether; R J Lewis |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of medical microbiology Volume: 38 ISSN: 0022-2615 ISO Abbreviation: J. Med. Microbiol. Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-04-16 Completed Date: 1993-04-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0224131 Medline TA: J Med Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 227-34 Citation Subset: IM |
Affiliation:
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Public Health Laboratory, Musgrove Park Hospital, Taunton, Somerset. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aeromonas
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metabolism,
pathogenicity* Animals Bacterial Toxins / biosynthesis, chemistry, immunology, isolation & purification* Cells, Cultured Chromatography, Gel Chromatography, Ion Exchange Cross Reactions Cyclic AMP / analysis, biosynthesis Electrophoresis, Polyacrylamide Gel Enterotoxins / biosynthesis, chemistry, immunology, isolation & purification* G(M1) Ganglioside / metabolism Hemolysin Proteins / biosynthesis, chemistry, immunology, isolation & purification* Humans Mice Molecular Weight Time Factors Virulence / physiology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 0/Enterotoxins; 0/Hemolysin Proteins; 37758-47-7/G(M1) Ganglioside; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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