| Isolation of pure populations of epithelial and myoepithelial cells from the normal human mammary gland using immunomagnetic separation with Dynabeads. | |
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MedLine Citation:
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PMID: 7785785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monoclonal antibodies to epithelial membrane antigen and common acute lymphoblastic leukemia antigen were bound to second antibody-coated magnetic microspheres. These specific antibody/bead complexes were then used to directly isolate purified epithelial and myoepithelial cells from normal breast organoid cell preparations by magnetic separation. Near homogeneous cell populations were selected with yields of 10-20 x 10(6) epithelial and myoepithelial cells per organoid preparation. Repeated purification steps allowed almost complete depletion of myoepithelial cells for RNA studies. Tissue culture of separated cell populations in appropriate defined media further ensured purity of cell type and was unimpeded by Dynabead attachment. |
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Authors:
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J J Gomm; P J Browne; R C Coope; Q Y Liu; L Buluwela; R C Coombes |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 226 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1995-07-20 Completed Date: 1995-07-20 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 91-9 Citation Subset: IM |
Affiliation:
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Department of Medical Oncology, Charing Cross and Westminster Medical School, University of London, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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immunology Base Sequence Breast / cytology* Cell Separation / methods* Cells, Cultured DNA, Complementary / genetics Electrophoresis, Agar Gel Epithelial Cells Female Gene Expression / genetics Humans Immunomagnetic Separation* Membrane Glycoproteins / immunology Molecular Sequence Data Mucin-1 Mucins / immunology Neprilysin / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/DNA, Complementary; 0/Membrane Glycoproteins; 0/Mucin-1; 0/Mucins; EC 3.4.24.11/Neprilysin |
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