Document Detail


Isolation, propagation and characterization of primary tubule cell culture from human kidney.
MedLine Citation:
PMID:  17371339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proximal tubule cells (PTC) are the major cell type in the cortical tubulointerstitium. Because PTC play a central role in tubulointerstitial pathophysiology, it is essential to prepare pure PTC from kidney tissue to explore the mechanisms of tubulointerstitial pathology. The authors have successfully refined and characterized primary cultures of human PTC using Percoll density gradient centrifugation as a key PTC enrichment step. The cells obtained by this method retain morphological and functional properties of PTC and are minimally contaminated by other renal cells. In particular, the primary isolates have characteristics of epithelial cells with uniform polarized morphology, tight junction and well-formed apical microvilli. Cytokeratin is uniformly and strongly expressed in the isolates. Brush border enzyme activities and PTC transport properties are retained in the isolates. This method therefore provides an excellent in vitro model for the physiologic study of the human proximal tubule.
Authors:
Weier Qi; David W Johnson; David A Vesey; Carol A Pollock; Xinming Chen
Related Documents :
11439099 - Polyamines regulate gap junction communication in connexin 43-expressing cells.
7550069 - Dye-coupling between term pregnant human myometrial cells before labor: carboxyfluoresc...
12045219 - Involvement of asip/par-3 in the promotion of epithelial tight junction formation.
16937189 - Inhibition of cardiomyocyte automaticity by electrotonic application of inward rectifie...
18835599 - Tunneling nanotubes (tnt) are induced by hiv-infection of macrophages: a potential mech...
20582379 - Band-gap tunable (cu2sn)(x/3)zn(1-x)s nanoparticles for solar cells.
24141049 - Identification of mmp-2 as a novel enhancer of cerebellar granule cell proliferation.
19269999 - Penta-o-galloyl-beta-d-glucose induces s- and g(1)-cell cycle arrests in prostate cance...
2164679 - Autocrine transformation by chimeric signal peptide-basic fibroblast growth factor: rev...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nephrology (Carlton, Vic.)     Volume:  12     ISSN:  1320-5358     ISO Abbreviation:  Nephrology (Carlton)     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-20     Completed Date:  2007-05-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9615568     Medline TA:  Nephrology (Carlton)     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  155-9     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, St Leonards, New South Wales, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Cell Culture Techniques
Cell Separation / methods*
Cell Shape
Cells, Cultured
Centrifugation, Density Gradient / methods
Epithelial Cells* / chemistry,  enzymology,  ultrastructure
Humans
Keratins / analysis
Kidney Tubules, Proximal / chemistry,  cytology*,  enzymology,  ultrastructure
Microvilli / enzymology,  ultrastructure
Povidone
Silicon Dioxide
Sodium-Hydrogen Antiporter / metabolism
Tight Junctions / ultrastructure
gamma-Glutamyltransferase / metabolism
Chemical
Reg. No./Substance:
0/Sodium-Hydrogen Antiporter; 65455-52-9/Percoll; 68238-35-7/Keratins; 7631-86-9/Silicon Dioxide; 9003-39-8/Povidone; EC 2.3.2.2/gamma-Glutamyltransferase; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of antihypertensive agents on plasma adiponectin levels in hypertensive patients with metabol...
Next Document:  Human peritoneal mesothelial cells isolated from spent dialysate fluid maintain contaminating macrop...