Document Detail

Isolation, propagation and characterization of primary tubule cell culture from human kidney.
MedLine Citation:
PMID:  17371339     Owner:  NLM     Status:  MEDLINE    
Proximal tubule cells (PTC) are the major cell type in the cortical tubulointerstitium. Because PTC play a central role in tubulointerstitial pathophysiology, it is essential to prepare pure PTC from kidney tissue to explore the mechanisms of tubulointerstitial pathology. The authors have successfully refined and characterized primary cultures of human PTC using Percoll density gradient centrifugation as a key PTC enrichment step. The cells obtained by this method retain morphological and functional properties of PTC and are minimally contaminated by other renal cells. In particular, the primary isolates have characteristics of epithelial cells with uniform polarized morphology, tight junction and well-formed apical microvilli. Cytokeratin is uniformly and strongly expressed in the isolates. Brush border enzyme activities and PTC transport properties are retained in the isolates. This method therefore provides an excellent in vitro model for the physiologic study of the human proximal tubule.
Weier Qi; David W Johnson; David A Vesey; Carol A Pollock; Xinming Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nephrology (Carlton, Vic.)     Volume:  12     ISSN:  1320-5358     ISO Abbreviation:  Nephrology (Carlton)     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-20     Completed Date:  2007-05-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9615568     Medline TA:  Nephrology (Carlton)     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  155-9     Citation Subset:  IM    
Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, St Leonards, New South Wales, Australia.
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MeSH Terms
Alkaline Phosphatase / metabolism
Cell Culture Techniques
Cell Separation / methods*
Cell Shape
Cells, Cultured
Centrifugation, Density Gradient / methods
Epithelial Cells* / chemistry,  enzymology,  ultrastructure
Keratins / analysis
Kidney Tubules, Proximal / chemistry,  cytology*,  enzymology,  ultrastructure
Microvilli / enzymology,  ultrastructure
Silicon Dioxide
Sodium-Hydrogen Antiporter / metabolism
Tight Junctions / ultrastructure
gamma-Glutamyltransferase / metabolism
Reg. No./Substance:
0/Sodium-Hydrogen Antiporter; 65455-52-9/Percoll; 68238-35-7/Keratins; 7631-86-9/Silicon Dioxide; 9003-39-8/Povidone; EC; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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