Document Detail


Isolation of polymorphic AGC repeats located 3' to bovine SINEs.
MedLine Citation:
PMID:  7985846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A bovine genomic library was screened for the presence of (AGC)n repeats. All isolated AGC repeats were located adjacent to the 3' end of bovine short interspersed nuclear elements (SINE). Polymerase chain reactions (PCR) using either two unique primers or one unique and one SINE primer produced high-resolution products without the secondary artifact ladders typical of dinucleotide microsatellites. Four AGC microsatellites were found to be polymorphic with 2-4 alleles each and polymorphism information context (PIC) values ranging between 0.26 and 0.49. One microsatellite, ARO25, was mapped to chromosome 26 with the CSIRO reference families. Because of their strong association with AGC repeats and high frequency in the genome, SINE-3' PCR may prove to be a novel source of polymorphic trinucleotide markers in the bovine genome.
Authors:
M Band; M Ron
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Animal genetics     Volume:  25     ISSN:  0268-9146     ISO Abbreviation:  Anim. Genet.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1995-01-03     Completed Date:  1995-01-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605704     Medline TA:  Anim Genet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  281-3     Citation Subset:  IM    
Affiliation:
Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U04801;  Z46598;  Z46599;  Z46600
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cattle / genetics*
DNA Primers
DNA, Satellite
Female
Male
Molecular Sequence Data
Pedigree
Polymerase Chain Reaction / veterinary
Polymorphism, Genetic*
Repetitive Sequences, Nucleic Acid*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Satellite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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