Document Detail

Isolation of pigment-free bulk lipids from thylakoids.
MedLine Citation:
PMID:  8418886     Owner:  NLM     Status:  MEDLINE    
Lipids from spinach thylakoids were extracted with chloroform/methanol and separated from pigments in a single chromatographic step run at 5 degrees C using silicic acid adjusted to pH 8. The isolated lipid fraction contained essentially the same amounts of individual lipids as in the initial extract. It contained less than 0.1% of the initial chlorophylls and carotenoids.
C E Bratt; H E Akerlund
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1165     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-02-10     Completed Date:  1993-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  288-90     Citation Subset:  IM    
Plant Biochemistry, University of Lund, Sweden.
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MeSH Terms
Chromatography / methods
Lipids / isolation & purification*
Pigments, Biological / isolation & purification
Plants / chemistry*
Silicic Acid
Reg. No./Substance:
0/Lipids; 0/Pigments, Biological; 1343-98-2/Silicic Acid

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