| Isolation of peroxisomes from tissues and cells by differential and density gradient centrifugation. | |
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MedLine Citation:
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PMID: 18228357 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peroxisome purification depends on a two-step procedure: differential centrifugation to prepare a light mitochondrial fraction and fractionation on a density-gradient medium preferably iodixanol or Nycodenz, to isolate the peroxisome enriched fraction. The iodixanol gradient may be a preformed continuous gradient or a self-generating gradient. Alternatively a continuous Nycodenz gradient or a simple Nycodenz barrier may be used for the second step. The unit contains protocols for peroxisome isolation from rat liver, tissue culture cells (HepG2 cells), and yeast spheroplasts. The extent of endoplasmic reticulum contamination of the prep can be assessed using an assay for the marker enzyme NADPH-cytochrome creductase. |
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Authors:
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J M Graham |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] Volume: Chapter 3 ISSN: 1934-2616 ISO Abbreviation: Curr Protoc Cell Biol Publication Date: 2001 May |
Date Detail:
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Created Date: 2008-01-29 Completed Date: 2008-03-24 Revised Date: 2009-12-11 |
Medline Journal Info:
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Nlm Unique ID: 101287856 Medline TA: Curr Protoc Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: Unit 3.5 Citation Subset: IM |
Affiliation:
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Liverpool John Moores University, Liverpool, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Carcinoma, Hepatocellular / pathology Cell Fractionation / methods* Cell Line, Tumor / ultrastructure Centrifugation / methods* Centrifugation, Density Gradient / methods Endoplasmic Reticulum / enzymology Humans Iohexol Liver / ultrastructure Liver Neoplasms / pathology NADPH-Ferrihemoprotein Reductase / analysis Peroxisomes* Rats Rats, Sprague-Dawley Saccharomyces cerevisiae / ultrastructure Spheroplasts / ultrastructure Triiodobenzoic Acids |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Triiodobenzoic Acids; 66108-95-0/Iohexol; 92339-11-2/iodixanol; EC 1.6.2.4/NADPH-Ferrihemoprotein Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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