Document Detail


Isolation and partial characterization of two glycoproteins from human liver metastases of sigmoid colon carcinoma.
MedLine Citation:
PMID:  2165927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Perchloric acid-soluble glycoprotein fraction (PASF) extracted from human liver metastases (LM) of sigmoid colon carcinoma was chromatographed on a DEAE-cellulose column. The main fraction (DEAE-nonadsorbed fraction) passed through the column was then subjected to Sephacryl S-200 superfine gel filtration and separated into 12 fractions. 2. Among 12 fractions, only both Fractions 3 and 4 were demonstrated to be chemically and immunologically homogeneous glycoproteins, respectively, by a combination of chemical composition analysis, SDS-PAGE and EITB assay using antisera against the DEAE-nonadsorbed fractions of PASFs from human LMs, normal liver (NL) and normal sigmoid colon (NSC). Each of Fractions 3 and 4 reacted with anti-LM serum to give one immuno complex on a nitrocellulose sheet in EITB assay, but did not react with anti-NL and -NSC sera. 3. Apparent molecular weights of 80,900 and 62,100, respectively, were found for Fractions 3 and 4. Both the fractions, respectively, had abnormal sugar compositions. Fraction 3 contained sialic acid, fucose, galactose, N-acetylglucosamine and N-acetylgalactosamine, but lacked glucose and mannose, and Fraction 4 contained sialic acid, fucose, galactose and N-acetylglucosamine, but lacked glucose, mannose and N-acetylgalactosamine, as sugar components.
Authors:
K Otsuka; S Ohkuma; T Nakajima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  22     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1990  
Date Detail:
Created Date:  1990-09-12     Completed Date:  1990-09-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  653-8     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
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MeSH Terms
Descriptor/Qualifier:
Glycoproteins / immunology,  isolation & purification*,  metabolism
Humans
Immunoblotting
Liver Neoplasms / analysis*,  secondary
Perchloric Acid
Sigmoid Neoplasms*
Chemical
Reg. No./Substance:
0/Glycoproteins; 7601-90-3/Perchloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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