| Isolation and partial characterization of two glycoproteins from human liver metastases of sigmoid colon carcinoma. | |
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MedLine Citation:
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PMID: 2165927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Perchloric acid-soluble glycoprotein fraction (PASF) extracted from human liver metastases (LM) of sigmoid colon carcinoma was chromatographed on a DEAE-cellulose column. The main fraction (DEAE-nonadsorbed fraction) passed through the column was then subjected to Sephacryl S-200 superfine gel filtration and separated into 12 fractions. 2. Among 12 fractions, only both Fractions 3 and 4 were demonstrated to be chemically and immunologically homogeneous glycoproteins, respectively, by a combination of chemical composition analysis, SDS-PAGE and EITB assay using antisera against the DEAE-nonadsorbed fractions of PASFs from human LMs, normal liver (NL) and normal sigmoid colon (NSC). Each of Fractions 3 and 4 reacted with anti-LM serum to give one immuno complex on a nitrocellulose sheet in EITB assay, but did not react with anti-NL and -NSC sera. 3. Apparent molecular weights of 80,900 and 62,100, respectively, were found for Fractions 3 and 4. Both the fractions, respectively, had abnormal sugar compositions. Fraction 3 contained sialic acid, fucose, galactose, N-acetylglucosamine and N-acetylgalactosamine, but lacked glucose and mannose, and Fraction 4 contained sialic acid, fucose, galactose and N-acetylglucosamine, but lacked glucose, mannose and N-acetylgalactosamine, as sugar components. |
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Authors:
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K Otsuka; S Ohkuma; T Nakajima |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The International journal of biochemistry Volume: 22 ISSN: 0020-711X ISO Abbreviation: Int. J. Biochem. Publication Date: 1990 |
Date Detail:
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Created Date: 1990-09-12 Completed Date: 1990-09-12 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0250365 Medline TA: Int J Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 653-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Tokyo College of Pharmacy, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Glycoproteins
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immunology,
isolation & purification*,
metabolism Humans Immunoblotting Liver Neoplasms / analysis*, secondary Perchloric Acid Sigmoid Neoplasms* |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 7601-90-3/Perchloric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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