| Isolation and partial characterization of a novel amino sugar-containing fucan sulfate from commercial Fucus vesiculosus fucoidan. | |
| | |
MedLine Citation:
|
PMID: 8181008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Commercial crude fucoidan (Sigma) from the brown seaweed Fucus vesiculosus was fractionated into its polysaccharide components by gel filtration and anion-exchange chromatography to clarify the structure-anticoagulant activity relationship. The products comprised a wide spectrum of fucans ranging from typical fucoidans (major components) containing mainly fucose, sulfate, and no uronic acid to low sulfate-containing heteropolysaccharide-like fucans (minor components) being composed of neutral sugars other than fucose and a high content of uronic acid(s). The polysaccharide components also had a wide range of molecular weight. The typical fucoidans showed a potent anticoagulant activity, whereas the other fucans had no or only slight activity. One of the fractions found as a minor component, was a novel polysaccharide containing an appreciable amount (11.5%) of glucosamine and a small amount (5.2%) of protein in addition to fucose and sulfate, and having a low apparent molecular weight of 6800. This is the first report that a proteoglycan-like, amino sugar-containing fucan sulfate, composed of fucose, galactose, glucose, mannose, xylose, uronic acid, glucosamine, and sulfate in the molar ratio of 1.00:0.04:0.01:0.48:0.24:0.18:0.56:1.90, could be obtained from brown seaweed. However, this polysaccharide showed no anticoagulant activity. |
| | |
Authors:
|
T Nishino; C Nishioka; H Ura; T Nagumo |
Related Documents
:
|
126088 - Alterations in the composition and bacteriophage-binding properties of walls of staphyl... 20355718 - Soluble polysaccharide composition and myo-inositol content help differentiate the anti... 811218 - Studies of polysaccharide fractions from the lipopolysaccharide of pseudomonas aerugino... 16660488 - Conversion of labeled substrates to sugars, cell wall polysaccharides, and tartaric aci... 8554388 - Cutaneous lipid synthesis during late fetal development in the rat. 5602578 - Antithromboplastic and thromboplastic activities of fatty acids. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Carbohydrate research Volume: 255 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 1994 Mar |
Date Detail:
|
Created Date: 1994-06-14 Completed Date: 1994-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 213-24 Citation Subset: IM |
Affiliation:
|
Department of Biophysics, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Algae, Brown
/
chemistry* Amino Sugars / chemistry* Anticoagulants / chemistry* Polysaccharides / chemistry* Proteoglycans / chemistry* Sulfuric Acid Esters |
| Chemical | |
Reg. No./Substance:
|
0/Amino Sugars; 0/Anticoagulants; 0/Polysaccharides; 0/Proteoglycans; 0/Sulfuric Acid Esters; 9072-19-9/fucoidan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular weight of low molecular weight heparins by 13C nuclear magnetic resonance spectroscopy.
Next Document: A sulfated alpha-L-fucan from sea cucumber.