| Isolation and partial characterization of a mucin-type glycoprotein from plasma membranes of human melanoma cells. | |
| | |
MedLine Citation:
|
PMID: 7284369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Plasma membranes were isolated from HM7 melanoma cells grown in the presence of [3H]glucosamine and Na2(35)SO4 or [3H]mannose and [14C]glucosamine. The labeled glycoconjugates were solubilized with 0.6 M lithium diiodosalicylate/0.5% Triton X-100. Fractionation of glycoconjugates by repeated chromatography on columns of Sepharose CL-6B and DEAE-Sepharose and by affinity chromatography on WGA-Sepharose yielded three radiochemically homogeneous glycoproteins. One of these having an apparent molecular weight of 100,000 was found to contain clusters of (AcNeu)1 or 2 leads to [Gal leads to GalNAc] linked O-glycosidically to the protein. One other glycoprotein contained both O-glycosidically and N-glycosidically-linked oligosaccharides, and the third contained only N-glycosidically-linked carbohydrates. Preliminary results indicate that the 100,000 molecular weight mucin-type glycoprotein is present in significantly reduced quantitites in cultured human fetal uveal melanocytes. Further, the bulk of the glycoproteins from the melanocytes were of lower molecular size compared to those from the melanoma cells. |
| | |
Authors:
|
J Umemoto; V P Bhavanandan; E A Davidson |
Related Documents
:
|
840339 - Human malignant melanoma cells: morphological and immunological variations. 3467129 - Gangliosides of human melanoma. 17289019 - Confluence-dependent resistance to doxorubicin in human mda-mb-231 breast carcinoma cel... 1562989 - Inhibitory effect of conjugated dienoic derivatives of linoleic acid and beta-carotene ... 20803329 - Modulation of 5-ala-induced ppix xenofluorescence intensities of a murine tumour and no... 2961439 - Surface antigens of human melanoma cells cultured in serum-free medium: induction of ex... 11017759 - Effect of reduced concentrations of glycerol and various macromolecules on the cryopres... 4059079 - The action spectrum for benzanthrone photosensitization of mouse macrophages. 15908029 - Influence of induction and operation mode on recombinant rhamnulose 1-phosphate aldolas... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 646 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1981 Sep |
Date Detail:
|
Created Date: 1981-12-15 Completed Date: 1981-12-15 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 402-10 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line Cell Membrane / analysis Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Female Glucosamine / metabolism Glycoproteins / isolation & purification* Humans Mannose / metabolism Melanoma / analysis* Molecular Weight Pregnancy |
| Grant Support | |
ID/Acronym/Agency:
|
CA 15483/CA/NCI NIH HHS; CA 17686/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Glycoproteins; 31103-86-3/Mannose; 3416-24-8/Glucosamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nocodazole inhibition of the vasopressin-induced water permeability increase in toad urinary bladder...
Next Document: Study on the dehydrating effect of the red cell Na+/K+-pump in nystatin-treated cells with varying N...