Document Detail


Isolation, partial characterization and mode of action of acidocin J1229, a bacteriocin produced by Lactobacillus acidophilus JCM 1229.
MedLine Citation:
PMID:  8972094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lactobacillus acidophilus JCM 1229 produces a heat-stable bacteriocin, designated as acidocin J1229, that has a narrow inhibitory spectrum. Production of acidocin J1229 in MRS broth was pH dependent, with maximum activity detected in broth culture maintained at pH 5.0. Acidocin J1229 was purified by ammonium sulphate precipitation and sequential cation exchange and reversed-phase chromatographies. The sequence of the first 24 amino acid residues of the N terminus of acidocin J1229 was determined. The molecular mass of acidocin J1229 as determined by mass spectrometry was 6301 Da. Acidocin J1229 showed a bactericidal effect but not a bacteriolytic effect on sensitive cells. Acidocin J1229 dissipated the membrane potential and the pH gradient in sensitive cells, which affected such proton motive force-dependent processes as amino acid transport. Acidocin J1229 also caused an efflux of glutamate, previously taken up via a unidirectional ATP-driven transport system. Secondary structure prediction revealed the presence of an amphiphilic alpha-helix region that could form hydrophilic pores. These results suggest that acidocin J1229 is a pore-forming peptide that creates cell membrane channels through the "barrel-stave' mechanism.
Authors:
T Tahara; K Kanatani
Related Documents :
14640574 - Tea polyphenols inhibit the transport of dietary phenolic acids mediated by the monocar...
18407564 - Nematicidal activity of anion transport blockers against meloidogyne incognita, caenorh...
15036654 - Expression and functional characterization of the system l amino acid transporter in kb...
10069984 - Cloning and characterization of a human electrogenic na+-hco-3 cotransporter isoform (h...
9747894 - Age-dependent telomere shortening is slowed down by enrichment of intracellular vitamin...
19571054 - In vitro enamel erosion associated with commercially available original-flavor and sour...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of applied bacteriology     Volume:  81     ISSN:  0021-8847     ISO Abbreviation:  J. Appl. Bacteriol.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-17     Completed Date:  1997-01-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7503050     Medline TA:  J Appl Bacteriol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  669-77     Citation Subset:  IM    
Affiliation:
Research Laboratory, Tamon Sake Brewing Co. Ltd, Hyogo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Anti-Bacterial Agents / pharmacokinetics
Bacterial Typing Techniques
Bacteriocins / chemistry*,  isolation & purification*,  pharmacology
Bacteriolysis
Cell Membrane / drug effects,  metabolism
Cell Membrane Permeability / drug effects,  physiology
Hydrogen-Ion Concentration
Lactobacillus acidophilus / drug effects,  growth & development,  metabolism*
Molecular Sequence Data
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacteriocins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The use of selective and differential agars in the isolation of Escherichia coli O157 from dairy her...
Next Document:  Note: isolation, characterization and epidemiology of Yersinia enterocolitica from humans and animal...