Document Detail

Isolation, partial characterization and mode of action of acidocin J1229, a bacteriocin produced by Lactobacillus acidophilus JCM 1229.
MedLine Citation:
PMID:  8972094     Owner:  NLM     Status:  MEDLINE    
Lactobacillus acidophilus JCM 1229 produces a heat-stable bacteriocin, designated as acidocin J1229, that has a narrow inhibitory spectrum. Production of acidocin J1229 in MRS broth was pH dependent, with maximum activity detected in broth culture maintained at pH 5.0. Acidocin J1229 was purified by ammonium sulphate precipitation and sequential cation exchange and reversed-phase chromatographies. The sequence of the first 24 amino acid residues of the N terminus of acidocin J1229 was determined. The molecular mass of acidocin J1229 as determined by mass spectrometry was 6301 Da. Acidocin J1229 showed a bactericidal effect but not a bacteriolytic effect on sensitive cells. Acidocin J1229 dissipated the membrane potential and the pH gradient in sensitive cells, which affected such proton motive force-dependent processes as amino acid transport. Acidocin J1229 also caused an efflux of glutamate, previously taken up via a unidirectional ATP-driven transport system. Secondary structure prediction revealed the presence of an amphiphilic alpha-helix region that could form hydrophilic pores. These results suggest that acidocin J1229 is a pore-forming peptide that creates cell membrane channels through the "barrel-stave' mechanism.
T Tahara; K Kanatani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of applied bacteriology     Volume:  81     ISSN:  0021-8847     ISO Abbreviation:  J. Appl. Bacteriol.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-17     Completed Date:  1997-01-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7503050     Medline TA:  J Appl Bacteriol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  669-77     Citation Subset:  IM    
Research Laboratory, Tamon Sake Brewing Co. Ltd, Hyogo, Japan.
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MeSH Terms
Amino Acid Sequence
Anti-Bacterial Agents / pharmacokinetics
Bacterial Typing Techniques
Bacteriocins / chemistry*,  isolation & purification*,  pharmacology
Cell Membrane / drug effects,  metabolism
Cell Membrane Permeability / drug effects,  physiology
Hydrogen-Ion Concentration
Lactobacillus acidophilus / drug effects,  growth & development,  metabolism*
Molecular Sequence Data
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacteriocins

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