Document Detail

Isolation and partial characterization of the major glycoprotein from the plasma membranes of AH-66 hepatoma cells.
MedLine Citation:
PMID:  465537     Owner:  NLM     Status:  MEDLINE    
A major glycoprotein of the plasma membranes of AH-66 hepatoma ascites cells was isolated in essentially pure form and in milligram amounts. The plasma membranes were solubilized with a solution containing both 0.3 M lithium diiodosalycylate and 0.2% cetylpyridinium chloride, and further extracted with 50% phenol, followed by gel filtration on Sepharose 6B in the presence of 0.1% Ammonyx-LO at pH 8.0. The apparent molecular weight of the purified glycoprotein was estimated to be 165 000 in 5.6% polyacrylamide gels, of which 54% was carbohydrate and 46% was protein. The chemical composition of the glycoprotein resembles glycophorin A from human erythrocyte membranes in that it has a high content of N-acetylgalactosamine, N-acetylglucosamine, galactose and sialic acid and a particularly large proportion of serine, threonine, aspartic acid and glutamic acid.
S Nakajo; K Nakaya; Y Nakamura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  579     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-10-26     Completed Date:  1979-10-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  88-94     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Cell Membrane / analysis
Electrophoresis, Polyacrylamide Gel
Glycoproteins / isolation & purification*
Liver Neoplasms, Experimental / analysis*
Membrane Proteins / isolation & purification*
Molecular Weight
Neoplasm Proteins / isolation & purification*
Reg. No./Substance:
0/Amino Acids; 0/Glycoproteins; 0/Membrane Proteins; 0/Neoplasm Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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