Document Detail

Isolation and partial characterization of the major glycoprotein from the plasma membranes of AH-66 hepatoma cells.
MedLine Citation:
PMID:  465537     Owner:  NLM     Status:  MEDLINE    
A major glycoprotein of the plasma membranes of AH-66 hepatoma ascites cells was isolated in essentially pure form and in milligram amounts. The plasma membranes were solubilized with a solution containing both 0.3 M lithium diiodosalycylate and 0.2% cetylpyridinium chloride, and further extracted with 50% phenol, followed by gel filtration on Sepharose 6B in the presence of 0.1% Ammonyx-LO at pH 8.0. The apparent molecular weight of the purified glycoprotein was estimated to be 165 000 in 5.6% polyacrylamide gels, of which 54% was carbohydrate and 46% was protein. The chemical composition of the glycoprotein resembles glycophorin A from human erythrocyte membranes in that it has a high content of N-acetylgalactosamine, N-acetylglucosamine, galactose and sialic acid and a particularly large proportion of serine, threonine, aspartic acid and glutamic acid.
S Nakajo; K Nakaya; Y Nakamura
Related Documents :
8285697 - Acylated proteins in borrelia hermsii, borrelia parkeri, borrelia anserina, and borreli...
2598637 - Water buffalo (bubalus bubalis) hemoglobins: an electrophoretic and chromatographic study.
3085687 - Acid-stable low molecular mass proteinase inhibitors in human lung lavage.
3873337 - The large sialoglycoprotein of human lymphocytes. ii. biochemical features.
15797237 - Cathepsin d-mediated yolk protein degradation is blocked by acid phosphatase inhibitors.
7766917 - Epr/spin-label technique as an analytical tool for determining the resistance of reacti...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  579     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-10-26     Completed Date:  1979-10-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  88-94     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / analysis
Cell Membrane / analysis
Electrophoresis, Polyacrylamide Gel
Glycoproteins / isolation & purification*
Liver Neoplasms, Experimental / analysis*
Membrane Proteins / isolation & purification*
Molecular Weight
Neoplasm Proteins / isolation & purification*
Reg. No./Substance:
0/Amino Acids; 0/Glycoproteins; 0/Membrane Proteins; 0/Neoplasm Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Separation of the integral membrane glycoproteins E1 and E2 of Semliki Forest virus by affinity chro...
Next Document:  Preparation of cyanogen bromide fragments of MM, NN, and MN glycoproteins (glycophorins) from human ...