Document Detail


Isolation of oligodendrocyte-like cells from human umbilical cord blood.
MedLine Citation:
PMID:  18608351     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: As human umbilical cord blood (UCB) is known to be a rich source of progenitor cells, the prospect of isolating a subset of these cells that could differentiate into cells of non-hematopoietic lineages suggests a therapeutic use for patients with inherited lysosomal and peroxisomal storage diseases currently treated with UCB transplantation. METHODS: Oligodendrocyte-like cells were isolated from UCB by density-gradient centrifugation and expanded using selective media. We then characterized this population of cells using standard immunohistochemical staining methods for neural cell proteins and polymerase chain reaction (PCR) to detect RNA sequences for myelin basic protein (MBP). We also developed a functional assay demonstrating myelination of neurons in vitro. RESULTS: Cells with oligodendrocyte-like morphology were reproducibly cultured ex vivo from fresh human UCB. Cells stained positively for multiple oligodendria cell markers (O1, MBP and CNPase) via immunohistochemical staining and flow cytometry. PCR confirmed the presence of MBP and CNPase mRNA. A further in vitro functional assay demonstrated the myelination of mature neuronal cells from the brain of a myelin-deficient murine model co-cultured with the oligodendrocyte-like cells. DISCUSSION: After human UCB transplant, donor-derived cells have been noted to migrate to the brain over time. Although is not known whether these cells solely deliver enzyme replacement or a subset engrafts and differentiates into mature neural cells, the clinical improvements noted in these patients suggest a potential role for targeted cellular therapy. Oligodendrocyte-like cells isolated ex vivo and expanded from human UCB could provide a potential cellular therapy for patients with demyelinating or dismyelinating diseases.
Authors:
E Tracy; J Aldrink; J Panosian; D Beam; J Thacker; M Reese; J Kurtzberg
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotherapy     Volume:  10     ISSN:  1477-2566     ISO Abbreviation:  Cytotherapy     Publication Date:  2008  
Date Detail:
Created Date:  2008-09-29     Completed Date:  2009-06-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100895309     Medline TA:  Cytotherapy     Country:  England    
Other Details:
Languages:  eng     Pagination:  518-25     Citation Subset:  IM    
Affiliation:
Pediatric Blood and Marrow Transplant Program, Duke University Medical Center, Durham, North Carolina 27710, USA.
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MeSH Terms
Descriptor/Qualifier:
2',3'-Cyclic-Nucleotide Phosphodiesterases / biosynthesis,  genetics,  immunology*
Animals
Antigens, Differentiation
Cell Culture Techniques
Cell Differentiation / immunology
Cell Lineage / immunology
Cell Separation
Centrifugation, Density Gradient
Female
Fetal Blood / cytology*,  metabolism
Humans
Immunohistochemistry
Mice
Mice, Knockout
Microscopy, Confocal
Myelin Proteins / biosynthesis,  genetics,  immunology*
Oligodendroglia / cytology*,  metabolism
Pregnancy
Chemical
Reg. No./Substance:
0/Antigens, Differentiation; 0/Myelin Proteins; EC 3.1.4.-/2',3'-Cyclic-Nucleotide Phosphodiesterases

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