Document Detail

Isolation of a novel mutant strain of Saccharomyces cerevisiae by an ethyl methane sulfonate-induced mutagenesis approach as a high producer of bioethanol.
MedLine Citation:
PMID:  18499058     Owner:  NLM     Status:  MEDLINE    
In order to obtain mutant strains showing higher bioethanol production than wild-type strains, a commercial Saccharomyces cerevisiae type was subjected to mutagenesis using ethyl methane sulfonate (EMS). After adding EMS to a shaken yeast suspension, the viability of yeast cells was assessed by diluted sample inoculation to solid yeast-extract peptone glucose (YEPG) medium at 15-min intervals. At 45 min, the viability of yeast cells was estimated to be about 40%. Mutagenized cells were recovered from YEPG broth after incubation at 30 degrees C for 18 h. After this period, EMS-treated yeast cells were grown on solid aerobic low-peptone (ALP) medium containing 2-12% (v/v) ethanol. All plates were incubated at 30 degrees C for 2-6 d in order to form colonies. The mutant strains that tolerated high concentrations of ethanol were selected for bioethanol production in microfuge tubes containing fermentation medium. Formation of bioethanol in small tubes was detected by the distillation-colorimetric method. In addition, trehalose content and invertase activity were determined in each mutant strain. Among many isolated mutant strains, there were six isolated colonies that grew on ALP medium supplemented with 10% (v/v) ethanol and one of them produced bioethanol 17.3% more than the wild type.
Mohsen Mobini-Dehkordi; Iraj Nahvi; Hamid Zarkesh-Esfahani; Kamran Ghaedi; Manoochehr Tavassoli; Rinji Akada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  105     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-23     Completed Date:  2008-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  403-8     Citation Subset:  IM    
Department of Biology, Faculty of Sciences, University of Isfahan, Azadi Sq., Daneshgah St., 81746-73441, Isfahan, I.R. Iran.
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MeSH Terms
Ethanol / metabolism*
Ethyl Methanesulfonate / pharmacology*
Industrial Microbiology*
Mutagenesis / drug effects*
Mutagens / pharmacology*
Saccharomyces cerevisiae / genetics*,  growth & development,  metabolism
Reg. No./Substance:
0/Mutagens; 62-50-0/Ethyl Methanesulfonate; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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