| Isolation of a new tautomerase monitored by the conversion of D-dopachrome to 5,6-dihydroxyindole. | |
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MedLine Citation:
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PMID: 8267597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two membrane bound enzymes which tautomerize L-dopachrome and are specific for the L-isomer of dopachrome have been defined in melanin forming cells. Another enzyme that tautomerizes D-dopachrome with concomitant decarboxylation to give 5,6-dihydroxyindole (DHI) was found in the cytoplasm of human melanoma cells, human liver and in all of the organs studied in rat. The decolorization of D-dopachrome with the formation of DHI was used in monitoring the isolation of a tautomerase from liver of male rats and therefore the enzyme is provisionally called D-dopachrome tautomerase. The molecular weight of D-dopachrome tautomerase monomer was approximately 12 kD and its N-terminal amino acid sequence was P-F-V-E-L-E-T-N-L-P-A-. The Km for D-dopachrome was 1.5 mM and Vmax 0.5 mmol per min and mg protein. |
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Authors:
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G Odh; A Hindemith; A M Rosengren; E Rosengren; H Rorsman |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 197 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-01-25 Completed Date: 1994-01-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 619-24 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Lund, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Brain / enzymology Chromatography, Gel Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Humans Indolequinones* Indoles / analysis, metabolism* Intramolecular Oxidoreductases* Isomerases / chemistry, isolation & purification*, metabolism* Kidney / enzymology Kinetics Liver / enzymology* Male Melanoma Molecular Sequence Data Molecular Weight Myocardium / enzymology Peptide Fragments / chemistry Quinones / metabolism* Rats Rats, Sprague-Dawley Sequence Homology, Amino Acid Spleen / enzymology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Indolequinones; 0/Indoles; 0/Peptide Fragments; 0/Quinones; 3131-52-0/5,6-dihydroxyindole; 3571-34-4/dopachrome; EC 5.-/Isomerases; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.3.12/dopachrome isomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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