Document Detail


Isolation of neurosecretory granules containing cytochromogenic hormone activity from the corpora cardiaca of Blaberus discoidalis cockroaches.
MedLine Citation:
PMID:  6628975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neurosecretory granules (NSG) were isolated by differential centrifugation from a homogenate of the corpora cardiaca-corpora allata complex of newly emerged, adult, female Blaberus discoidalis cockroaches. By centrifuging at 11,000 g, the procedure yielded a final pellet that was enriched in small, membrane-bound vesicles (103 nm mean diameter). This pellet contained 61% of the cytochromogenic activity present in the original homogenate at an 11-fold increase in concentration. A pellet formed at 4000 g contained a mixture of mitochondria and larger NSG (146 nm mean diameter) and most of the hindgut-stimulating hormone activity. This procedure provided a quick, simple method for purifying neurosecretory granules and separating them into two categories of size and endocrine content.
Authors:
E Lawson-Smith; T K Hayes; L L Keeley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  52     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1983-12-20     Completed Date:  1983-12-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  149-56     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fractionation / methods
Cockroaches / anatomy & histology,  physiology*
Cytochromes / biosynthesis
Cytoplasmic Granules / physiology,  ultrastructure
Female
Insect Hormones / isolation & purification*
Mitochondria / physiology
Neurosecretory Systems / ultrastructure*
Grant Support
ID/Acronym/Agency:
AI 15190/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Cytochromes; 0/Insect Hormones

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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