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Isolation of neural stem cells from neural tissues using the neurosphere technique.
MedLine Citation:
PMID:  21049474     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
This unit describes protocols for the derivation, characterization, and expansion of neural stem cell (NSC) lines from the adult mouse subventricular zone (mNSCs), embryonic mouse brain and from the human fetal brain (hNSCs). NSCs can be isolated by enzymatic digestion of specific regions (NSCs niches) of the central nervous system (CNS) and grown in suspension. By using this methodology, NSCs form spherical clusters called neurospheres, which are mechanically dissociated to a single-cell suspension and replated in the selective culture medium. Removal of growth factors and plating cells on an adherent substrate allows cells to differentiate into neurons, astrocytes, and oligodendrocytes, the main cell type of the CNS. Correct culturing of NSCs, according to this methodology, will allow cells to expand over 100 passages without alteration of cell karyotype, growth ability, and differentiation potential.
Authors:
Daniela Ferrari; Elena Binda; Lidia De Filippis; Angelo Luigi Vescovi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in stem cell biology     Volume:  Chapter 2     ISSN:  1938-8969     ISO Abbreviation:  Curr Protoc Stem Cell Biol     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101470226     Medline TA:  Curr Protoc Stem Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit2D.6     Citation Subset:  IM    
Copyright Information:
© 2010 by John Wiley & Sons, Inc.
Affiliation:
Department of Biotechnology and Biosciences, University Milan-Bicocca, Milan, Italy.
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