| Isolation and morphofunctional characterization of mussel digestive gland cells in vitro. | |
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MedLine Citation:
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PMID: 9127736 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to isolate and characterize digestive gland cells of the bivalve mollusc Mytilus galloprovincialis Lmk. The digestive gland of bivalves is a complex organ composed of digestive and connective tissues but it is also invaded by the reproductive tissue as gametogenesis proceeds. The digestive tissue is comprised of stomach and intestinal epithelial cells, ciliated and non-ciliated duct cells, digestive cells and basophilic cells. the last two cell types are found lining the epithelium of the blind-ending digestive tubules and are the main focus of this study. Two different approaches were assayed for cell isolation, i.e., explant culture techniques and mechanical plus enzymatical digestion techniques. Cell viability was tested by trypan blue exclusion, neutral red uptake and ultrastructural analysis. The explant cultures were often contaminated with bacteria and spermatozoa and, moreover, cells migrating out of the explants possibly corresponded to hemocytes and not to digestive tissue cells. Mechanical plus enzymatical digestion with collagenase was concluded to be the method of choice for digestive cell isolation, with a percentage of about 30% of isolated cells corresponding to digestive cells. The ultrastructure of digestive cells isolated with the latter procedure closely resembled that of mussel digestive cells in vivo. |
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Authors:
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Y Robledo; M P Cajaraville |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of cell biology Volume: 72 ISSN: 0171-9335 ISO Abbreviation: Eur. J. Cell Biol. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-08-12 Completed Date: 1997-08-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7906240 Medline TA: Eur J Cell Biol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 362-9 Citation Subset: IM |
Affiliation:
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Biologia Zelularra eta Zientzia Morfologikoen Saila, Euskal Herriko Unibertsitatea, Universidad del País Vasco, Bilbo, Basque Country, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bivalvia / ultrastructure* Cell Separation / methods* Cell Survival Digestive System / ultrastructure Epithelium / ultrastructure Microscopy, Electron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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