| Isolation of monoclonal antibodies against avian oncornaviral protein p19. | |
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MedLine Citation:
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PMID: 6268819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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For the production of monoclonal antibodies against pp60src and the gag precursor protein Pr76gag, the spleens of mice bearing tumors that had been induced by avian sarcoma virus Schmidt-Ruppin D-transformed cells were used. One hybridoma culture produced antibodies that were directed against the p19 portion of the gag precursor. However, no antibodies directed against pp60src could be detected in any of the hybridoma supernatants. The anti-p19-producing hybridoma culture was cloned twice in soft agar, and a stable clone was used for the production of high-titer ascites fluid in mice. The monoclonal antibodies belonged to the immunoglobulin G subclass 2b. The antibodies precipitated Pr76gag and the processed virion-associated p19, as well as the 75,000-molecular-weight gag fusion protein from avian erythroblastosis virus-transformed bone marrow cells. Also, viral ribonucleoprotein complexes were specifically precipitable, indicating that they contain p19 molecules. |
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Authors:
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I Greiser-Wilke; K M Owada; K Moelling |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of virology Volume: 39 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1981 Jul |
Date Detail:
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Created Date: 1981-11-18 Completed Date: 1981-11-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 325-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alpharetrovirus
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immunology* Animals Antibodies, Monoclonal / isolation & purification* Antibodies, Viral / isolation & purification Cell Transformation, Neoplastic Cell Transformation, Viral Clone Cells Hybrid Cells Immunoglobulin G / isolation & purification Mice Protein Precursors / immunology Viral Core Proteins Viral Proteins / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Immunoglobulin G; 0/Protein Precursors; 0/Viral Core Proteins; 0/Viral Proteins |
| Comments/Corrections | |
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