| Isolation of mono-caffeoylquinic acids from tobacco waste using continuous resin-based pre-separation and preparative HPLC. | |
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MedLine Citation:
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PMID: 22733520 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Three isomers of mono-caffeoylquinic acid, specifically, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid and 5-O-caffeoylquinic acid, were successfully isolated from a crude extract of tobacco (Nicotiana tobaccum L.) wastes using continuous resin-based pre-separation and preparative high-performance liquid chromatography (HPLC). The extract of tobacco wastes was continuously pre-separated by resin-based columns packed with D101 and XAD-4, yielding total mono-caffeoylquinic acids with a purity of 67.71% and a recovery rate of 90.06%. Variables affecting resolution and productivity of three mono-caffeoylquinic acid isomers in preparative HPLC (i.e. mobile-phase composition, pH, flow rate and loading amount) were studied. The optimum chromatographic conditions were determined to be a mobile phase consisting of 15% (v/v) methanol and aqueous acetic acid with a pH of 4.5, a flow rate of 4.0 mL/min, a loading amount of 4 mL and a detection wavelength of 360 nm. From 300 mg of loading sample, 56.3 mg of 3-O-caffeoylquinic acid, 92.8 mg of 5-O-caffeoylquinic acid and 73.1 mg of 4-O-caffeoylquinic acid were obtained in a single run, each with a purity of over 98% by HPLC. The structures of the isolated compounds were elucidated by ESI-MS, (1) H-NMR and (13) C-NMR spectral data. |
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Authors:
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Jun Wang; Dingqiang Lu; Yao Liang; Hui Zhao; Min Luo; Xiuquan Ling; Pingkai Ouyang |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of separation science Volume: 35 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-06-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 1379-87 Citation Subset: IM |
Copyright Information:
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© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, P. R. China; School of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, P. R. China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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