Document Detail


Isolation and molecular identification of Cronobacter spp. from powdered infant formula (PIF) in Bangladesh.
MedLine Citation:
PMID:  20685000     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cronobacter spp. formerly known as Enterobacter sakazakii is an occasional contaminant of powdered infant formula (PIF). This pathogen has been associated with out-breaks of a rare form of infant meningitis, necrotizing enterocolitis (NEC), bacteremia and neonate deaths. The organism is ranked by the International Commission for Microbiological Specifications for Foods (ICMSF) as a 'Severe hazard for restricted populations, life threatening or substantial chronic sequelae or long duration'. Present study aimed to isolate Cronobacter spp. from PIF and clinical samples, such as blood, stool and CSF collected from 93 neonates and child patients, age ranged from 0 to 24months. We did not detect Cronobacter spp. in any of these samples. Later 32 PIF samples collected from retail markets in Bangladesh were tested for the presence of Cronobacter spp. Of these only one was found to be contaminated with Cronobacter sp. This is the first case of Cronobacter contaminated PIF found in Bangladesh to be reported. The organism was successfully identified based on its typical culture characteristics, producing blue-green colonies on chromogenic DFI agar and also by a standardized conventional PCR assay targeting the alpha glucosidase and 16S rRNA gene sequence of Cronobacter sp. The 16S rRNA gene was partially sequenced to provide for the phylogenetic analysis of this isolate (DA01) and found to cluster with some other Cronobacter isolates in the phylogram.
Authors:
Ashfaqul Hoque; Tahmeed Ahmed; Mohammad Shahidullah; Anowar Hossain; Abdul Mannan; Khaled Noor; Kamrun Nahar; Mohammad Ilias; Dilruba Ahmed
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-16
Journal Detail:
Title:  International journal of food microbiology     Volume:  142     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  375-8     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/GQ918494
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MeSH Terms
Descriptor/Qualifier:
Bangladesh
Base Sequence
Cluster Analysis
Consumer Product Safety
Enterobacter sakazakii / classification,  isolation & purification*
Feces / microbiology
Food Contamination / analysis*
Food Microbiology
Humans
Infant
Infant Food / analysis,  microbiology*
Infant Formula*
Infant, Newborn
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Chemical
Reg. No./Substance:
0/RNA, Bacterial; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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