Document Detail


Isolation and molecular characterization of a H5N1 virus isolated from a Jungle crow (Corvus macrohynchos) in India.
MedLine Citation:
PMID:  20396941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In 2008, India experienced widespread outbreaks of H5N1 virus in West Bengal, Tripura, and Assam. The virus was detected in Kamrup district of Assam in November 2008 and subsequently spread to eight more districts. Two Jungle or Large billed crows (Corvus macrohynchos) were found dead in a hospital campus at about 8 km from the foci of initial detection of the virus in the same district. One of the crows was positive for H5N1 avian influenza virus by virus isolation, real time RT-PCR, and RT-PCR tests. Full length sequencing of all the eight segments of the virus was carried out. The phylogenetic analysis indicated that all the eight genes grouped with clade 2.2 viruses and were closely related to the human isolate of Bangladesh and avian isolates from India, Bangladesh, Kuwait, Germany, and Saudi Arabia. The molecular analysis indicated avian receptor (alpha 2,3 sialic acid) specificity, susceptibility to oseltamivir and amantadine group of antivirals and lower pathogenicity to mice.
Authors:
S Nagarajan; C Tosh; H V Murugkar; G Venkatesh; M Katare; R Jain; P Behera; R Khandia; S Tripathi; D D Kulkarni; S C Dubey
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-16
Journal Detail:
Title:  Virus genes     Volume:  41     ISSN:  1572-994X     ISO Abbreviation:  Virus Genes     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-17     Completed Date:  2010-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803967     Medline TA:  Virus Genes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  30-6     Citation Subset:  IM    
Affiliation:
High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh, 462 021, India. nagavetbio@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Crows
India
Influenza A Virus, H5N1 Subtype / genetics*,  isolation & purification
Influenza in Birds / virology*
Phylogeny

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