Document Detail

Isolation of merozoite rhoptries, identification of novel rhoptry-associated proteins from Plasmodium yoelii, P. chabaudi, P. berghei, and conserved interspecies reactivity of organelles and proteins with P. falciparum rhoptry-specific antibodies.
MedLine Citation:
PMID:  9676705     Owner:  NLM     Status:  MEDLINE    
Rhoptries were isolated from merozoites of P. yoelii (17 XL), P. chabaudi adami and P. berghei (K-173), using sucrose gradient density centrifugation. Mouse antisera was prepared against the organelles and characterized. Antibodies specific for a known P. yoelii rhoptry protein were used to identify gradient fractions containing rhoptries and electron microscopy was used to confirm rhoptry enrichment and organelle morphology. Western blotting analysis of the gradients with organelle-specific antisera from each species, revealed several major cross-reactive interspecies protein bands of approximately 235, 210, 180, 160/170, 140, and 96-110 kDa, predominantly in densities of 1.12 and 1.15 g/ml. The parasite origin of the proteins was verified by immunoprecipitation, and reactive epitopes localized to the rhoptries by IEM. By Western blotting antisera specific for P. falciparum rhoptries reacted with protein bands of approximately 96-110 kDa in schizont extracts, and gradient fractions of density 1.12 and 1.15 g/ml from all three rodent malaria species, as well as with the rhoptries in P. yoelii, P. chabaudi, and P. berghei merozoites by IEM. We conclude that the three rodent malaria species and P. falciparum share conserved interspecies epitopes.
T Y Sam-Yellowe; R A Del Rio; H Fujioka; M Aikawa; J C Yang; Z Yakubu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental parasitology     Volume:  89     ISSN:  0014-4894     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-07-24     Completed Date:  1998-07-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  271-84     Citation Subset:  IM    
Department of Biology, Cleveland State University, Ohio 44115, USA.
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MeSH Terms
Antibodies, Monoclonal / immunology
Antibodies, Protozoan / immunology
Antigens, Protozoan / analysis*,  chemistry,  immunology
Blotting, Western
Cell Fractionation
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
Epitopes / analysis,  chemistry,  immunology
Fluorescent Antibody Technique, Indirect
Immune Sera / immunology
Microscopy, Immunoelectron
Molecular Weight
Organelles / chemistry,  immunology,  ultrastructure
Plasmodium berghei / chemistry*,  immunology,  ultrastructure
Plasmodium chabaudi / chemistry*,  immunology,  ultrastructure
Plasmodium yoelii / chemistry*,  immunology,  ultrastructure
Precipitin Tests
Protozoan Proteins / analysis*,  chemistry,  immunology
Silver Staining
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Protozoan; 0/Antigens, Protozoan; 0/Epitopes; 0/Immune Sera; 0/Protozoan Proteins; 0/rhoptry associated protein, Plasmodium

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