Document Detail

Isolation and measurement of 15N2 from respiratory gases of animals administered 15N-labeled substances.
MedLine Citation:
PMID:  7328697     Owner:  NLM     Status:  MEDLINE    
A method is described for collection of metabolic 15N2 from in vitro preparations or intact rats administered 15N-containing compounds. The methods enables routine collection and mass spectrometric measurement of as little as 10 mumol 15N2 respired by a rat over a 24-h period. A device is described that includes either an animal chamber or a tissue reaction vessel in a closed recycling atmosphere, with automatic O2 replenishment and removal of CO2 and water. It is capable of sustaining moderate vacuum and is coupled to a high-vacuum manifold designed to process the contained atmosphere and respiratory gases. The starting atmosphere is an 80:20 mix of sulfur hexafluoride and O2. Recovery of 15N2 gas from the system without an animal present was 101.3 +/- 5.75%. When 15N2 gas was very slowly infused iv into an animal, recovery was 89.1 +/- 5.38%. Use of the method in studies of the fate of [15N]hydrazine in rats indicated that about 15% of the administered hydrazine is rapidly converted to 15N2, followed by slower conversion of an additional 7-10% over the next several hours.
D L Springer; D J Reed; F N Dost
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of toxicology and environmental health     Volume:  8     ISSN:  0098-4108     ISO Abbreviation:  J Toxicol Environ Health     Publication Date:    1981 Jul-Aug
Date Detail:
Created Date:  1982-04-20     Completed Date:  1982-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7513622     Medline TA:  J Toxicol Environ Health     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11-9     Citation Subset:  IM; S    
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MeSH Terms
Atmosphere Exposure Chambers
Mass Spectrometry
Nitrogen / analysis,  metabolism*
Nitrogen Isotopes
Rats, Inbred Strains
Respiratory System / metabolism*
Reg. No./Substance:
0/Nitrogen Isotopes; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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