Document Detail


Isolation and mapping of 75 new DNA markers on human chromosome 3.
MedLine Citation:
PMID:  1674499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated and mapped 75 new DNA markers including 52 restriction fragment length polymorphism (RFLP) markers on human chromosome 3. Clones were mapped by nonisotopic in situ hybridization, in which discrete fluorescent signals can be detected on prometaphase R-banded chromosomes. Thirty-seven markers were mapped to each arm of chromosome 3, and one was localized to the centromere. Five markers defined variable number of tandem repeat (VNTR) loci. Although the 75 clones were scattered throughout the chromosome, they were concentrated in the R-positive bands. This physical map of chromosome 3 will contribute to the characterization of the chromosomal and molecular aberrations involved in renal cell carcinoma, small-cell lung cancer, and other malignancies and in single-gene disorders such as von Hippel-Lindau disease and autosomal dominant retinitis pigmentosa.
Authors:
K Yamakawa; E Takahashi; H Saito; T Sato; M Oshimura; T Hori; Y Nakamura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  9     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-06-25     Completed Date:  1991-06-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  536-43     Citation Subset:  IM    
Affiliation:
Division of Biochemistry, Cancer Institute, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blotting, Southern
Cell Line
Centromere
Chromosome Aberrations
Chromosome Banding
Chromosome Mapping*
Chromosomes, Human, Pair 3*
Genes, Dominant
Genes, Lethal
Genetic Markers*
Humans
Hybrid Cells
Metaphase
Molecular Sequence Data
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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