Document Detail

Isolation of macrophages from human placenta.
MedLine Citation:
PMID:  3981018     Owner:  NLM     Status:  MEDLINE    
Human placentae have been extracted with combinations of enzymes to optimize the release of mononuclear phagocytes. A mixture of trypsin-DNAase used in sequential extraction was found to provide the best yield of adherent cells which were stable in culture. The majority of adherent cells exhibited phagocytic function and expression of receptor for IgG-Fc (FcR). Subsequent studies established that these functions were co-expressed by the same cells. The FcR+ cells were also shown by immunofluorescence with monoclonal antibodies to display monocyte-macrophage distinctive antigens and class I and class II MHC antigens. The placenta has thus been shown to provide a rich source of class II-positive macrophages suitable for immunological studies.
S Uren; W Boyle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunological methods     Volume:  78     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1985 Apr 
Date Detail:
Created Date:  1985-05-15     Completed Date:  1985-05-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  25-34     Citation Subset:  IM    
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MeSH Terms
Antigens, Surface / analysis
Cell Adhesion
Culture Media
Macrophages / cytology*
Monocytes / immunology
Placenta / cytology*,  immunology
Receptors, Fc / analysis
Reg. No./Substance:
0/Antigens, Surface; 0/Culture Media; 0/Receptors, Fc

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