Document Detail


Isolation and localization of a slow troponin (TnT) gene on chromosome 19 by subtraction hybridization of a cDNA muscle library using myotonic dystrophy muscle cDNA.
MedLine Citation:
PMID:  1706783     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Subtraction hybridization techniques were used to isolate 91 cDNA clones which are overexpressed in normal control skeletal muscle relative to muscle from patients with myotonic muscular dystrophy. The gene responsible for myotonic dystrophy (DM) has been localized to the 19q13.2-13.3 region of chromosome 19. To test as a candidate gene for DM, clones which represent differences in transcription are analyzed for localization to chromosome 19. One clone, designated MSL 366, was found to be on the long arm of chromosome 19 distal to the CKMM gene at 19q13.2. Sequence analysis confirmed that MSL 366 is the cDNA for human slow skeletal muscle troponin T. A genomic clone has been isolated and linkage studies with DM are in progress.
Authors:
F Samson; J E Lee; W Y Hung; T G Potter; M Herbstreith; A D Roses; J R Gilbert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  27     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-04-26     Completed Date:  1991-04-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  441-51     Citation Subset:  IM    
Affiliation:
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Blotting, Southern
Chromosome Mapping
Chromosomes, Human, Pair 19*
DNA / analysis,  isolation & purification*
DNA-Directed DNA Polymerase / metabolism
Gene Library
Genomic Library
Humans
Molecular Sequence Data
Muscles / metabolism*
Muscular Dystrophies / genetics*
Nucleic Acid Hybridization
Plasmids
RNA / metabolism
Troponin / genetics*
Grant Support
ID/Acronym/Agency:
AG07922/AG/NIA NIH HHS; M01-RR-30/RR/NCRR NIH HHS; NS19999/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Troponin; 63231-63-0/RNA; 9007-49-2/DNA; EC 2.7.7.7/DNA-Directed DNA Polymerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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