| Isolation of lipase producing thermophilic bacteria: Optimization of production and reaction conditions for lipase from Geobacillus sp. | |
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MedLine Citation:
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PMID: 23195552 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Lipases catalyze the hydrolysis and the synthesis of esters formed from glycerol and long chain fatty acids. Lipases occur widely in nature, but only microbial lipases are commercially significant. In the present study, thirty-two bacterial strains, isolated from soil sample of a hot spring were screened for lipase production. The strain TS-4, which gave maximum activity, was identified as Geobacillus sp. at MTCC, IMTECH, Chandigarh. The isolated lipase producing bacteria were grown on minimal salt medium containing olive oil. Maximal quantities of lipase were produced when 30 h old inoculum was used at 10% (v/v) in production medium and incubated in shaking conditions (150 rpm) for 72 h. The optimal temperature and pH for the bacterial growth and lipase production were found to be 60°C and 9.5, respectively. Maximal enzyme production resulted when mustard oil was used as carbon source and yeast extract as sole nitrogen source at a concentration of 1% (v/v) and 0.15% (w/v), respectively. The different optimized reaction parameters were temperature 65°C, pH 8.5, incubation time 10 min and substrate p-nitrophenyl palmitate. The Km and Vmax values of enzyme were found to be 14 mM and 17.86 μmol ml-1min-1, respectively, with p-nitrophenyl palmitate as substrate. All metal ions studied (1 mM) increased the lipase activity. |
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Authors:
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Akshita Mehta; Rakesh Kumar; Reena Gupta |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta microbiologica et immunologica Hungarica Volume: 59 ISSN: 1217-8950 ISO Abbreviation: Acta Microbiol Immunol Hung Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-11-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9434021 Medline TA: Acta Microbiol Immunol Hung Country: Hungary |
Other Details:
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Languages: eng Pagination: 435-50 Citation Subset: IM |
Affiliation:
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Himachal Pradesh University Department of Biotechnology Summer Hill Shimla 171005 India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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