Document Detail


Isolation of lipase producing thermophilic bacteria: Optimization of production and reaction conditions for lipase from Geobacillus sp.
MedLine Citation:
PMID:  23195552     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Lipases catalyze the hydrolysis and the synthesis of esters formed from glycerol and long chain fatty acids. Lipases occur widely in nature, but only microbial lipases are commercially significant. In the present study, thirty-two bacterial strains, isolated from soil sample of a hot spring were screened for lipase production. The strain TS-4, which gave maximum activity, was identified as Geobacillus sp. at MTCC, IMTECH, Chandigarh. The isolated lipase producing bacteria were grown on minimal salt medium containing olive oil. Maximal quantities of lipase were produced when 30 h old inoculum was used at 10% (v/v) in production medium and incubated in shaking conditions (150 rpm) for 72 h. The optimal temperature and pH for the bacterial growth and lipase production were found to be 60°C and 9.5, respectively. Maximal enzyme production resulted when mustard oil was used as carbon source and yeast extract as sole nitrogen source at a concentration of 1% (v/v) and 0.15% (w/v), respectively. The different optimized reaction parameters were temperature 65°C, pH 8.5, incubation time 10 min and substrate p-nitrophenyl palmitate. The Km and Vmax values of enzyme were found to be 14 mM and 17.86 μmol ml-1min-1, respectively, with p-nitrophenyl palmitate as substrate. All metal ions studied (1 mM) increased the lipase activity.
Authors:
Akshita Mehta; Rakesh Kumar; Reena Gupta
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta microbiologica et immunologica Hungarica     Volume:  59     ISSN:  1217-8950     ISO Abbreviation:  Acta Microbiol Immunol Hung     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434021     Medline TA:  Acta Microbiol Immunol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  435-50     Citation Subset:  IM    
Affiliation:
Himachal Pradesh University Department of Biotechnology Summer Hill Shimla 171005 India.
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