| Isolation of intact collagen fibrils from healing ligament. | |
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MedLine Citation:
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PMID: 9345777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The inability to isolate intact collagen fibrils has limited the study of their growth and structure. Although intact fibrils have been isolated from echinoderms and from embryonic chick tissues, no method has previously succeeded in isolating intact collagen fibrils from a postfoetal vertebrate tissue. Having previously observed that gentamicin weakens rat tail tendon, we hypothesized that gentamicin may weaken interfibrillar bonds and that intact collagen fibrils might be isolated from tissue treated with gentamicin. In this study medial collateral knee ligaments of Sprague Dawley rats were transected and then harvested 24, 48 or 96 h postoperatively. These specimens were placed in gentamicin or phosphate-buffered saline for 72 h, vortexed for 1 h, incubated in gentamicin for an additional 24 h, and vortexed again for 1 h. Negatively stained specimens were examined with a transmission electron microscope. The phosphate-buffered saline specimens yielded only broken fibrils. The gentamicin specimens yielded both broken and intact fibrils. The latter and tapering ends and consisted of molecules orientated such that their amino termini pointed toward the tip and their carboxy termini pointed toward a short central region where the molecular polarity reversed. |
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Authors:
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J E deVente; G E Lester; J A Trotter; L E Dahners |
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Publication Detail:
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Type: Letter; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of electron microscopy Volume: 46 ISSN: 0022-0744 ISO Abbreviation: J Electron Microsc (Tokyo) Publication Date: 1997 |
Date Detail:
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Created Date: 1997-12-08 Completed Date: 1997-12-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7611157 Medline TA: J Electron Microsc (Tokyo) Country: JAPAN |
Other Details:
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Languages: eng Pagination: 353-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Collagen / chemistry, isolation & purification*, ultrastructure* Gentamicins Medial Collateral Ligament, Knee / chemistry*, drug effects, injuries Microscopy, Electron Rats Rats, Sprague-Dawley Wound Healing |
| Chemical | |
Reg. No./Substance:
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0/Gentamicins; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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