| Isolation of individual capsaicinoids from a mixture and their characterization by (13)C NMR spectrometry. | |
| | |
MedLine Citation:
|
PMID: 18970769 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Individual compounds were isolated from a laboratory mixture of capsaicinoids by a multi-stage approach. First, the capsaicinoids were fractionated into capsaicins and non-capsaicins by argentation solid phase extraction (SPE) on a silver-charged propyl sulfonate resin. Second, compounds in each fraction were isolated by semi-preparative liquid chromatography on a C(30) phase in aqueous methanol. Third, the individual components of the original mixture were concentrated by reversed phased (C(18)) SPE. The structure of each purified compound was confirmed by (13)C NMR spectrometry and spectral comparison to known standards, purchased or synthesized locally. The chemical shifts of 15 capsaicinoid standards were measured on a 600MHz instrument, and their assignments to particular carbons were made by reference to Distortionless Enhancement by Polarization Transfer (DEPT) NMR experiments and NMR spectral prediction software. |
| | |
Authors:
|
Robert Q Thompson; Michael J Pennino; Michael J Brenner; Manish A Mehta |
Related Documents
:
|
19289049 - Development of a cp 31p nmr broadline simulation methodology for studying the interacti... 18021739 - Solid-state 2h nmr spectroscopy of retinal proteins in aligned membranes. 12633829 - 35cl quadrupolar constants obtained by solid-state nmr: study of chlorinated al-o-p clu... 12481689 - The importance of being ordered: improving nmr structures using residual dipolar coupli... 15700639 - Chromones from licania arianeae (chrysobalanaceae). 19150229 - A (59)co nmr study to observe the effects of ball milling on small ferromagnetic cobalt... 17441109 - Metallomacrocycles that incorporate cofacially aligned diimide units. 21582719 - {bis[2-(diphenyl-phosphino)eth-yl]phenyl-phosphine-κp,p',p''}chloridopalladium(ii) hex... 15835869 - Inorganic-organic hybrid molecular architectures of cyanometalate host and organic gues... |
Publication Detail:
|
Type: Journal Article Date: 2006-03-24 |
Journal Detail:
|
Title: Talanta Volume: 70 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2006 Sep |
Date Detail:
|
Created Date: 2008-10-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
|
Languages: eng Pagination: 315-22 Citation Subset: - |
Affiliation:
|
119 Woodland Street, Department of Chemistry and Biochemistry, Oberlin College, Oberlin, OH 44074, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Application of factorial design in optimization of ultrasonic-assisted extraction of aluminum in jui...
Next Document: A new kinetic method for quantification phenoxyl free radicals.