| Isolation and in vitro cultivation turns cells from exocrine human pancreas into multipotent stem-cells. | |
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MedLine Citation:
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PMID: 19716277 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several research groups have reported on the existence and in vitro characterization of multipotent stem-cells from the pancreas. However, the origin of these cells remains largely unexplained. Here, we report that in vitro culturing itself can turn adult cells from human exocrine pancreas into a cell population with typical stem cell characteristics. A simple, yet reliable method enabled us to track cell fates: Combining automated continuous observation using time-lapse microscopy with immunocytochemical analyses, we found that a significant fraction of the pancreatic cells ( approximately 14%) can survive trypsination and displays a drastic change in the protein expression profile. After further cultivation, these cells give rise to a heterogeneous cell population with typical multipotent stem cell characteristics; i.e. they proliferate over long time periods and continuously give rise to specialized cells from at least two germ layers. Although we cannot exclude that a rare pre-existing stem cell-type also contributes to the final in vitro-population, the majority of cells must have been arisen from mature pancreatic cells. Our findings indicate that multipotent cells for regenerative medicine, instead of being laboriously isolated, can be generated in large amounts by in vitro de-differentiation. |
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Authors:
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Daniel H Rapoport; Simone Schicktanz; Emel G??rleyik; Christine Z??hlke; Charli Kruse |
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Publication Detail:
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Type: Journal Article Date: 2009-07-21 |
Journal Detail:
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Title: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft Volume: 191 ISSN: 1618-0402 ISO Abbreviation: Ann. Anat. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-09-14 Completed Date: 2010-01-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100963897 Medline TA: Ann Anat Country: Germany |
Other Details:
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Languages: eng Pagination: 446-58 Citation Subset: IM |
Affiliation:
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Fraunhofer Institute of Marine Biotechnology, Paul-Ehrlich-Strasse 1-3, 23562 L??beck, Germany. daniel.rapoport@emb.fraunhofer.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Biological Markers / analysis Biopsy Cell Culture Techniques / methods Cell Differentiation / physiology Cell Division / physiology Cell Separation / methods Cell Survival / physiology Chromosome Banding / methods Humans Immunohistochemistry / methods Kinetics Male Middle Aged Pancreas, Exocrine / cytology*, drug effects, pathology Pancreatic Neoplasms / pathology*, surgery Pluripotent Stem Cells / cytology*, drug effects Trypsin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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