Document Detail


Isolation and identification of inulooligosaccharides resulting from inulin hydrolysis.
MedLine Citation:
PMID:  17983784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, inulooligosaccharides (F(n)-type inulin) resulting from the endo-inulinase hydrolysis of globe artichoke inulin were purified and characterized. The aim was to produce F(n) oligomer standards with the intention of identifying them in the complex inulin chromatogram. Inulin was extracted from globe artichoke and presented a high average degree of polymerization (DP) of about 80 as determined by high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). This inulin was hydrolyzed by a commercial endo-inulinase yielding a product with a very high F(n)/GF(n) molecule ratio, thus limiting the interference of GF(n) during the purification process. High-performance size exclusion chromatography was used to individually isolate and collect each retention peak corresponding to a specific oligomer. The purity of these fractions was checked by HPAEC-PAD and showed that relatively pure molecules were produced. Electrospray ionization mass spectrometry allowed the molecular weight determination of these purified oligomers and ascertained their DP as F2, F3 and F4. These F(2-4) standards were used with glucose, fructose, sucrose and GF(2-4) (commercially available) to spike commercial oligofructose products in order to determine the elution profile in the HPAEC-PAD chromatogram.
Authors:
Sébastien N Ronkart; Christophe S Blecker; Hélène Fourmanoir; Christian Fougnies; Claude Deroanne; Jean-Claude Van Herck; Michel Paquot
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Publication Detail:
Type:  Journal Article     Date:  2007-08-06
Journal Detail:
Title:  Analytica chimica acta     Volume:  604     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-06     Completed Date:  2007-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  81-7     Citation Subset:  IM    
Affiliation:
Department of Food Technology, Gembloux Agricultural University, Passage des Déportés 2, B-5030 Gembloux, Belgium. ronkart.s@fsagx.ac.be
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Electrochemistry
Hydrolysis
Inulin / chemistry*
Oligosaccharides / chemistry*
Spectrometry, Mass, Electrospray Ionization
Chemical
Reg. No./Substance:
0/Oligosaccharides; 9005-80-5/Inulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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