Document Detail

Isolation and identification of cellulose-binding proteins from sheep rumen contents.
MedLine Citation:
PMID:  19151184     Owner:  NLM     Status:  MEDLINE    
To extend our understanding of the mechanisms of plant cell wall degradation in the rumen, cellulose-binding proteins (CBPs) from the contents of a sheep rumen were directly isolated and identified using a metaproteomics approach. The rumen CBPs were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and some CBPs revealed endoglucanase activities toward carboxymethyl cellulose. Using mass spectrometry analyses, four CBPs were identified and annotated as known proteins from the predominant rumen cellulolytic bacterium Fibrobacter succinogenes: tetratricopeptide repeat domain protein, OmpA family protein, fibro-slime domain protein, and cellulose-binding endoglucanase F (EGF). Another CBP was identified as the cellulosomal glycosyl hydrolase family 6 exoglucanase, Cel6A, of Piromyces equi. F. succinogenes cells expressing EGF were found to be major members of the bacterial community on the surface or at the inner surface of hay stems by immunohistochemical analyses using anti-EGF antibody. The finding that four of the five CBPs isolated and identified from sheep rumen contents were from F. succinogenes indicates that F. succinogenes is significantly involved in cellulose degradation in the rumen.
Atsushi Toyoda; Wataru Iio; Makoto Mitsumori; Hajime Minato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-16
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  75     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-09     Completed Date:  2009-05-15     Revised Date:  2010-09-23    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1667-73     Citation Subset:  IM    
College of Agriculture, Ibaraki University, Ami, Ibaraki 300-0393, Japan.
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MeSH Terms
Bacterial Proteins / isolation & purification,  metabolism
Cellulose / metabolism*
Electrophoresis, Polyacrylamide Gel
Fibrobacter / enzymology*
Fungal Proteins / isolation & purification,  metabolism
Piromyces / enzymology*
Protein Binding
Proteins / isolation & purification*,  metabolism*
Rumen / chemistry*
Reg. No./Substance:
0/Bacterial Proteins; 0/Fungal Proteins; 0/Proteins; 9004-34-6/Cellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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