Document Detail

Isolation and identification of cancer stem-like cells in esophageal carcinoma cell lines.
MedLine Citation:
PMID:  18680391     Owner:  NLM     Status:  MEDLINE    
Side population (SP) cells may play an important role in tumorigenesis and cancer therapy. We isolate and identify the cancer stem-like cells in human esophageal carcinoma (EC) cell lines, EC9706 and EC109 cells labeled with Hoechst 33342. Both the cell lines contained SP cells, and the cells that had the strongest dye efflux activity ("Tip"-SP cell) in EC9706 had higher clone formation efficiency than non-SP cells. When transplanted into nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, "Tip"-SP cells showed at least 50 times higher tumorigenicity than non-SP cells. Microarray analysis discriminated a differential gene expression profile between "Tip"-SP and non-SP cells, which is further tested using quantitative real-time RT-PCR. It is ascertained that several important stem cell-related genes (including OCT-4, SOX-2, BMI-1, and ZFX), two ATP-binding cassette (ABC) transporter genes (ABCG2 and ABCA5), and three Wnt and two Notch signal pathway-related genes (such as FZD10, PTGS2, KLF5, TTK, and RBM15) were upregulated in "Tip"-SP cells. Western blot showed a higher expression of beta-catenin protein in "Tip"-SP cells. All these indicated that the minority population described as "Tip"-SP cells possessed cancer stem cell character. Further understanding of tumor stem cell-specific traits will offer insights on the early stages of tumorigenesis for prevention and enhanced selectivity of antitumor therapeutics.
Dingzhi Huang; Quanli Gao; Liping Guo; Chunpeng Zhang; Wei Jiang; Hongxia Li; Jing Wang; Xiaohong Han; Yuankai Shi; Shih Hsin Lu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells and development     Volume:  18     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-20     Completed Date:  2009-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  465-73     Citation Subset:  IM    
Department of Etiology and Carcinogenesis, Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
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MeSH Terms
ATP-Binding Cassette Transporters / genetics,  metabolism
Benzimidazoles / metabolism
Cell Line, Tumor*
Drug Resistance, Neoplasm
Esophageal Neoplasms / pathology*
Fluorescent Dyes / metabolism
Gene Expression Profiling
Membrane Proteins / genetics,  metabolism
Mice, Inbred NOD
Mice, Nude
Mice, SCID
Microarray Analysis
Neoplasm Proteins / genetics,  metabolism
Neoplasm Transplantation
Neoplastic Stem Cells / cytology*,  physiology
Reproducibility of Results
Transplantation, Heterologous
beta Catenin / metabolism
Reg. No./Substance:
0/ABCG2 protein, human; 0/ATP-Binding Cassette Transporters; 0/Benzimidazoles; 0/CKAP4 protein, human; 0/Fluorescent Dyes; 0/Membrane Proteins; 0/Neoplasm Proteins; 0/beta Catenin; 23491-52-3/HOE 33342

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