| Isolation and identification of cancer stem-like cells in esophageal carcinoma cell lines. | |
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MedLine Citation:
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PMID: 18680391 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Side population (SP) cells may play an important role in tumorigenesis and cancer therapy. We isolate and identify the cancer stem-like cells in human esophageal carcinoma (EC) cell lines, EC9706 and EC109 cells labeled with Hoechst 33342. Both the cell lines contained SP cells, and the cells that had the strongest dye efflux activity ("Tip"-SP cell) in EC9706 had higher clone formation efficiency than non-SP cells. When transplanted into nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, "Tip"-SP cells showed at least 50 times higher tumorigenicity than non-SP cells. Microarray analysis discriminated a differential gene expression profile between "Tip"-SP and non-SP cells, which is further tested using quantitative real-time RT-PCR. It is ascertained that several important stem cell-related genes (including OCT-4, SOX-2, BMI-1, and ZFX), two ATP-binding cassette (ABC) transporter genes (ABCG2 and ABCA5), and three Wnt and two Notch signal pathway-related genes (such as FZD10, PTGS2, KLF5, TTK, and RBM15) were upregulated in "Tip"-SP cells. Western blot showed a higher expression of beta-catenin protein in "Tip"-SP cells. All these indicated that the minority population described as "Tip"-SP cells possessed cancer stem cell character. Further understanding of tumor stem cell-specific traits will offer insights on the early stages of tumorigenesis for prevention and enhanced selectivity of antitumor therapeutics. |
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Authors:
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Dingzhi Huang; Quanli Gao; Liping Guo; Chunpeng Zhang; Wei Jiang; Hongxia Li; Jing Wang; Xiaohong Han; Yuankai Shi; Shih Hsin Lu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Stem cells and development Volume: 18 ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-06-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: United States |
Other Details:
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Languages: eng Pagination: 465-73 Citation Subset: IM |
Affiliation:
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Department of Etiology and Carcinogenesis, Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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genetics,
metabolism Animals Benzimidazoles / metabolism Cell Line, Tumor* Drug Resistance, Neoplasm Esophageal Neoplasms / pathology* Fluorescent Dyes / metabolism Gene Expression Profiling Humans Membrane Proteins / genetics, metabolism Mice Mice, Inbred NOD Mice, Nude Mice, SCID Microarray Analysis Neoplasm Proteins / genetics, metabolism Neoplasm Transplantation Neoplastic Stem Cells / cytology*, physiology Reproducibility of Results Transplantation, Heterologous beta Catenin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/ABCG2 protein, human; 0/ATP-Binding Cassette Transporters; 0/Benzimidazoles; 0/CKAP4 protein, human; 0/Fluorescent Dyes; 0/Membrane Proteins; 0/Neoplasm Proteins; 0/beta Catenin; 23491-52-3/HOE 33342 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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