| Isolation and identification by gas chromatographic mass spectrometry of the carbonyl-active site of pig kidney diamine oxidase. | |
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MedLine Citation:
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PMID: 7978244 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An adduct with phenylhydrazine was formed with the purified pig kidney diamine oxidase and in parallel with the l-tyrosine decarboxylase from Streptococcus faecalis. The labeled enzymes were hydrolyzed by chemical hydrolysis and the adducts released by hydrolysis were isolated and identified first in HPLC and successively in GC-MS. Both enzymes gave the same adduct which was identified as the phenylhydrazone of pyridoxal. The isolated adduct had the same retention time as the phenylhydrazone of pyridoxal in HPLC and in gas chromatography and showed the same molecular weight in mass spectrometry when chemical ionization was used and the same fragmentation in mass spectrometry when electronic impact was used. The reported results show that pig kidney diamine oxidase contains pyridoxal in the form of covalently linked pyridoxal phosphate which can be released from the enzyme only by chemical hydrolysis. Pig kidney diamine oxidase is therefore a pyridoxal enzyme such as l-tyrosine decarboxylase as hypothesized in the past but never clearly demonstrated. |
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Authors:
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F Buffoni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 220 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-12-12 Completed Date: 1994-12-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 185-91 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Florence, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amine Oxidase (Copper-Containing)
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analysis* Animals Binding Sites Chromatography, High Pressure Liquid Enterococcus faecalis / enzymology Gas Chromatography-Mass Spectrometry* Kidney / enzymology* Phenylhydrazines / chemistry Pyridoxal Phosphate / analysis Spectrophotometry Spectrophotometry, Ultraviolet Swine Tyrosine Decarboxylase / analysis |
| Chemical | |
Reg. No./Substance:
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0/Phenylhydrazines; 100-63-0/phenylhydrazine; 54-47-7/Pyridoxal Phosphate; EC 1.4.3.6/Amine Oxidase (Copper-Containing); EC 4.1.1.25/Tyrosine Decarboxylase |
| Comments/Corrections | |
Erratum In:
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Anal Biochem 1994 Nov 1;222(2):520 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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