| Isolation of the hypertrehalosaemic factors I and II from the corpus cardiacum of the Indian stick insect, Carausius morosus, by reversed-phase high-performance liquid chromatography, and amino-acid composition of factor II. | |
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MedLine Citation:
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PMID: 3986046 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The separation of two peptides with hypertrehalosaemic activity from the corpus cardiacum of the Indian stick insect, Carausius morosus, was achieved by reversed-phase high-performance liquid chromatography using a Nucleosil C-18 column with a trifluoroacetic acid/acetonitrile gradient. The eluant was monitored at 210 nm and the hypertrehalosaemic activity was detected in ligated stick insects using a bioassay. In addition, the isolated material was potent in causing hyperlipaemia in migratory locusts and also in raising blood carbohydrates in the American cockroach. The amino-acid composition of the major peptide, hypertrehalosaemic factor II, was determined after acid hydrolysis with HCl or methanesulfonic acid. The analyses suggest that factor II is a nonapeptide which contains the following amino-acid residues: Asp, 2 Thr, Glu, Pro, Gly, Leu, Phe and Trp. This composition is almost identical to that of locust adipokinetic hormone I, lacking only one Asp residue. |
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Authors:
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G Gäde |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological chemistry Hoppe-Seyler Volume: 366 ISSN: 0177-3593 ISO Abbreviation: Biol. Chem. Hoppe-Seyler Publication Date: 1985 Feb |
Date Detail:
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Created Date: 1985-06-19 Completed Date: 1985-06-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8503054 Medline TA: Biol Chem Hoppe Seyler Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 195-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Chromatography, High Pressure Liquid Disaccharides / analysis* Hemolymph / analysis Insect Hormones / analysis, isolation & purification* Insects / analysis* Oligopeptides* Periplaneta / metabolism Species Specificity Trehalose / analysis* Tryptophan / analysis |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Disaccharides; 0/Insect Hormones; 0/Oligopeptides; 0/hypertrehalosaemic factor (Carausius morosus); 53027-55-7/adipokinetic hormone (locust); 73-22-3/Tryptophan; 99-20-7/Trehalose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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