Document Detail


Isolation of the hypertrehalosaemic factors I and II from the corpus cardiacum of the Indian stick insect, Carausius morosus, by reversed-phase high-performance liquid chromatography, and amino-acid composition of factor II.
MedLine Citation:
PMID:  3986046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The separation of two peptides with hypertrehalosaemic activity from the corpus cardiacum of the Indian stick insect, Carausius morosus, was achieved by reversed-phase high-performance liquid chromatography using a Nucleosil C-18 column with a trifluoroacetic acid/acetonitrile gradient. The eluant was monitored at 210 nm and the hypertrehalosaemic activity was detected in ligated stick insects using a bioassay. In addition, the isolated material was potent in causing hyperlipaemia in migratory locusts and also in raising blood carbohydrates in the American cockroach. The amino-acid composition of the major peptide, hypertrehalosaemic factor II, was determined after acid hydrolysis with HCl or methanesulfonic acid. The analyses suggest that factor II is a nonapeptide which contains the following amino-acid residues: Asp, 2 Thr, Glu, Pro, Gly, Leu, Phe and Trp. This composition is almost identical to that of locust adipokinetic hormone I, lacking only one Asp residue.
Authors:
G Gäde
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological chemistry Hoppe-Seyler     Volume:  366     ISSN:  0177-3593     ISO Abbreviation:  Biol. Chem. Hoppe-Seyler     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-06-19     Completed Date:  1985-06-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503054     Medline TA:  Biol Chem Hoppe Seyler     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  195-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Chromatography, High Pressure Liquid
Disaccharides / analysis*
Hemolymph / analysis
Insect Hormones / analysis,  isolation & purification*
Insects / analysis*
Oligopeptides*
Periplaneta / metabolism
Species Specificity
Trehalose / analysis*
Tryptophan / analysis
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Disaccharides; 0/Insect Hormones; 0/Oligopeptides; 0/hypertrehalosaemic factor (Carausius morosus); 53027-55-7/adipokinetic hormone (locust); 73-22-3/Tryptophan; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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