Document Detail


Isolation of human simple repeat loci by hybridization selection.
MedLine Citation:
PMID:  8069306     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated short tandem repeat arrays from the human genome, using a rapid method involving filter hybridization to enrich for tri- or tetranucleotide tandem repeats. About 30% of clones from the enriched library cross-hybridize with probes containing trimeric or tetrameric tandem arrays, facilitating the rapid isolation of large numbers of clones. In an initial analysis of 54 clones, 46 different tandem arrays were identified. Analysis of these tandem repeat loci by PCR showed that 24 were polymorphic in length; substantially higher levels of polymorphism were displayed by the tetrameric repeat loci isolated than by the trimeric repeats. Primary mapping of these loci by linkage analysis showed that they derive from 17 chromosomes, including the X chromosome. We anticipate the use of this strategy for the efficient isolation of tandem repeats from other sources of genomic DNA, including DNA from flow-sorted chromosomes, and from other species.
Authors:
J A Armour; R Neumann; S Gobert; A J Jeffreys
Related Documents :
14627806 - Mutagenic stress modulates the dynamics of ctg repeat instability associated with myoto...
8581556 - Origins and evolution of huntington disease chromosomes.
8587876 - Human minisatellite loci composed of interspersed gga-ggt triplet repeats.
20306126 - Genome organization of major tandem repeats in the hard tick, ixodes scapularis.
11164336 - Effect of age and sex on the production of internal and external hydrocarbons and phero...
1303176 - An x chromosome inactivation assay based on differential methylation of a cpg island co...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human molecular genetics     Volume:  3     ISSN:  0964-6906     ISO Abbreviation:  Hum. Mol. Genet.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-09-27     Completed Date:  1994-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9208958     Medline TA:  Hum Mol Genet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  599-65     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Leicester, UK.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X73214;  X73215;  X73287;  X73288;  X73289;  X73290;  X73291;  X73967;  X73968;  X73969;  X73970;  X74431;  X74432;  X74433;  X74434;  X74435;  X74436;  X74778;  X74779;  X74780;  X74781;  X75409;  X75550;  X75551;  X75552;  X75553;  X75554;  X75555;  X75556;  X76102
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA / genetics*
Genome, Human*
Humans
Molecular Sequence Data
Nucleic Acid Hybridization*
Polymerase Chain Reaction
Polymorphism, Genetic*
Repetitive Sequences, Nucleic Acid*
Sequence Alignment
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Detailed mapping of germline deletions of the von Hippel-Lindau disease tumour suppressor gene.
Next Document:  A deletion of 1.6 kb proximal to the CGG repeat of the FMR1 gene causes the clinical phenotype of th...