Document Detail


Isolation of human myeloid dendritic cells from tumor tissue and peripheral blood.
MedLine Citation:
PMID:  16634524     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Human myeloid dendritic cells (MDC) have been identified in human solid tumor tissue of head and neck squamous cell carcinoma (HNSCC), their cellular functions being strongly affected in this environment. The characterization of MDC differentiation and functions requires the establishment of highly efficient isolation procedures. MATERIALS AND METHODS: Human MDC were isolated from peripheral blood and solid HNSCC using density gradient centrifugation or preparation of single cell suspensions, respectively. The cells were isolated by "magnetic bead separation" using magnetically-labelled antibodies and were analyzed by flow cytometry. RESULTS: The isolation of human MDC from peripheral blood or solid HNSCC tissue resulted in highly enriched cell populations and revealed cell purities of about 90%. CONCLUSION: The scale of molecular investigations on human myeloid dendritic cells is restricted by the limited proportion of this cell type. Thus, highly efficient and gentle MDC isolation procedures are essential to characterize in vivo matured MDC.
Authors:
Carsten Brocks; Hendrik Graefe; Henning Frenzel; Ralph Pries; Barbara Wollenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  20     ISSN:  0258-851X     ISO Abbreviation:  In Vivo     Publication Date:    2006 Mar-Apr
Date Detail:
Created Date:  2006-04-25     Completed Date:  2006-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  239-42     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, University of Schleswig-Holstein Campus Lübeck, 23538 Lübeck, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Carcinoma, Squamous Cell / pathology*
Cell Separation / methods*
Centrifugation, Density Gradient
Dendritic Cells / cytology*
Feasibility Studies
Flow Cytometry / methods*
Head and Neck Neoplasms / pathology*
Humans
Immunomagnetic Separation
Middle Aged
Myeloid Cells / cytology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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