| Isolation of high-rate DNA synthetic cells by con A chromatography. | |
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MedLine Citation:
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PMID: 565783 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The separation of L1210 cells with columns of Con A-derivatized nylon was investigated. Most of the cells bound to the column irreversibly. The binding was lectin-specific. Cells were pulse labeled with 3H-thymidine and applied to Con A columns. Those cells not binding the columns were enriched in incorporated thymidine compared to the unseparated population. Data is presented which suggests that a small, synchronized fraction of cells synthesizing DNA at a high rate is reduced in Con A-nylon affinity. It is proposed that L1210 cell DNA synthesis is not uniform in rate and that changes in this rate are related to changes in the ability of cells to bind Con A-nylon. |
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Authors:
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M A Wallenbrock; J J Killion |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 95 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1978 May |
Date Detail:
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Created Date: 1978-07-15 Completed Date: 1978-07-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 151-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Cell Line Cell Separation / methods Chromatography, Affinity / methods Concanavalin A DNA, Neoplasm / biosynthesis* Leukemia L1210 / metabolism* Nylons Thymidine / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Nylons; 11028-71-0/Concanavalin A; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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