| Isolation of high density lipoprotein subclasses by electrofiltration and their chemical components. | |
| | |
MedLine Citation:
|
PMID: 19431042 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The exact role of high density lipoprotein in atheroprotection is not well understood yet due to its complex nature; it comprises more than ten subclasses that vary in size, composition, and function. Isolation and characterization of these subclasses is an important step for further studies addressing their functions in health and disease. In this work, we present a novel method that is relatively simple and efficient for isolation of high density lipoprotein subclasses. The method depends on fractional filtration of the subclasses through a preformed gel membrane system under the effect of an electric field, where the stepwise isolation of the subclasses depends on differences in their rates of migration in polyacrylamide gel. Using this design, we were able to isolate seven high density lipoprotein subclasses with relative molecular masses of 42,000-50,000; 71,000; 103,000; 124,000; 150,000; 182,000; and 219,000. All the subclasses contained apolipoprotein A-I, phosphatidylcholine, sphingomyelin, free cholesterol, esterified cholesterol, and triacylglycerols. Some fractions of some samples contained the apolipoproteins A-II, C-I, C-II, C-III, and E. A subclass of molecular mass of 106,000 was identified and isolated from a healthy young subject that contained albumin and apoA-I with some free and esterified cholesterol, but with no triacylglycerols. This electrofiltration technique offers a novel tool for isolating pure native high density lipoprotein subclasses in a concentrated form that can be used directly for detailed studies of their physicochemical and physiological properties. |
| | |
Authors:
|
Ragheb F Atmeh; Belal M Kana'an; Tariq T Massad |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Preparative biochemistry & biotechnology Volume: 39 ISSN: 1532-2297 ISO Abbreviation: Prep. Biochem. Biotechnol. Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-05-11 Completed Date: 2009-08-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9607037 Medline TA: Prep Biochem Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 248-65 Citation Subset: IM |
Affiliation:
|
Department of Chemical Sciences, Jordan University of Science and Technology, Irbid, Jordan. ragheb@just.edu.jo |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, Thin Layer Electrochemistry / methods* Electrophoresis, Polyacrylamide Gel Filtration / methods* Lipoproteins, HDL / classification, isolation & purification* |
| Chemical | |
Reg. No./Substance:
|
0/Lipoproteins, HDL |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protein depletion using IgY from chickens immunised with human protein cocktails.
Next Document: Novozyme 435-catalyzed asymmetric acylation of (R, S)-3-n- butylphthalide in hexane.