Document Detail

Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica.
MedLine Citation:
PMID:  2890654     Owner:  NLM     Status:  MEDLINE    
Entamoeba histolytica adheres to human colonic mucus, colonic epithelial cells, and other target cells via a galactose (Gal) or N-acetyl-D-galactosamine (GalNAc) inhibitable surface lectin. Blockade of this adherence lectin with Gal or GalNAc in vitro prevents amebic killing of target cells. We have identified and purified the adherence lectin by two methods: affinity columns derivatized with galactose monomers or galactose terminal glycoproteins, and affinity columns and immunoblots prepared with monoclonal antibodies that inhibit amebic adherence. By both methods the adherence lectin was identified as a 170-kD secreted and membrane-bound amebic protein. The surface location of the lectin was confirmed by indirect immunofluorescence. Purified lectin competitively inhibited amebic adherence to target cells by binding to receptors on the target Chinese hamster ovary cells in a Gal-inhibitable manner.
W A Petri; R D Smith; P H Schlesinger; C F Murphy; J I Ravdin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  80     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1987-12-23     Completed Date:  1987-12-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1238-44     Citation Subset:  AIM; IM    
Department of Medicine, University of Virginia, Charlottesville 22908.
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MeSH Terms
Antibodies, Monoclonal
Binding, Competitive
Cell Adhesion
Cell Line
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Entamoeba histolytica / physiology*
Fluorescent Antibody Technique
Hemagglutinins / isolation & purification*,  metabolism
Immunoenzyme Techniques
Immunologic Techniques
Orosomucoid / analogs & derivatives
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Asialoglycoproteins; 0/Galectins; 0/Hemagglutinins; 0/Orosomucoid; 0/asialoorosomucoid; 26566-61-0/Galactose

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