| Isolation and functional characterization of proinflammatory acidic phospholipase A(2) from Bothrops leucurus snake venom. | |
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MedLine Citation:
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PMID: 21699995 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In the present study, an acidic PLA(2), designated Bl-PLA(2), was isolated from Bothrops leucurus snake venom through two chromatographic steps: ion-exchange on CM-Sepharose and hydrophobic chromatography on Phenyl-Sepharose. Bl-PLA(2) was homogeneous on SDS-PAGE and when submitted to 2D electrophoresis the molecular mass was 15,000Da and pI was 5.4. Its N-terminal sequence revealed a high homology with other Asp49 acidic PLA(2)s from snake venoms. Its specific activity was 159.9U/mg and the indirect hemolytic activity was also higher than that of the crude venom. Bl-PLA(2) induced low myotoxic and edema activities as compared to those of the crude venom. Moreover, the enzyme was able to induce increments in IL-12p40, TNF-α, IL-1β and IL-6 levels and no variation of IL-8 and IL-10 in human PBMC stimulated in vitro, suggesting that Bl-PLA(2) induces proinflammatory cytokine production by human mononuclear cells. Bothrops leucurus venom is still not extensively explored and knowledge of its components will contribute for a better understanding of its action mechanism. |
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Authors:
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Débora C O Nunes; Renata S Rodrigues; Malson N Lucena; Camila T Cologna; Ana Carolina S Oliveira; Amélia Hamaguchi; Maria I Homsi-Brandeburgo; Eliane C Arantes; David N S Teixeira; Carlos U Vieira; Veridiana M Rodrigues |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-15 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP Volume: - ISSN: 1532-0456 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100959500 Medline TA: Comp Biochem Physiol C Toxicol Pharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
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Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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