Document Detail

Isolation from human serum of an inactivator of bacterial lipopolysaccharide.
MedLine Citation:
PMID:  70173     Owner:  NLM     Status:  MEDLINE    
By a series of chromatographic procedures involving precipitation by salt, gel filtration, anionic exchange, and hydroxyapatite elution, a protein--termed the lipopolysaccharide inactivator (LPS-I)--has been isolated from normal human serum. As a result of treatment of bacterial lipopolysaccharide (LPS) by LPS-I, the treated LPS loses its toxicity for mice and reactivity in the Limulus assay and appears to be irreversibly disaggregated. The inactivation of the LPS by the purified LPS-I is temperature and time dependent and is not blocked by the addition of irreversible inhibitors of serine esterases. The LPS inactivator migrates as an alpha-globulin in whole serum and has a sedimentation velocity of approximately 4.5S. Characteristics of the inactivated LPS are briefly described using internally labeled LPS.
K J Johnson; P A Ward; S Goralnick; M J Osborn
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of pathology     Volume:  88     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1977-09-29     Completed Date:  1977-09-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  559-74     Citation Subset:  AIM; IM    
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MeSH Terms
Alpha-Globulins / isolation & purification
Blood Protein Electrophoresis
Blood Proteins / isolation & purification*
Chromatography, Gel
Endotoxins / antagonists & inhibitors
Horseshoe Crabs
Lipopolysaccharides / antagonists & inhibitors*
Polysaccharides, Bacterial / antagonists & inhibitors*
Time Factors
Reg. No./Substance:
0/Alpha-Globulins; 0/Blood Proteins; 0/Endotoxins; 0/Hydroxyapatites; 0/Lipopolysaccharides; 0/Polysaccharides, Bacterial

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