Document Detail


Isolation from human placenta of the IgG transporter, FcRn, and localization to the syncytiotrophoblast: implications for maternal-fetal antibody transport.
MedLine Citation:
PMID:  8871627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The IgG transporter responsible for ferrying maternal IgG across the human placenta to fetal circulation has not been identified, although the human homologue of the neonatal rat Fc receptor (FcRn), a heterodimer with pH-dependent IgG affinity, structurally similar to MHC Class I molecules, was recently proposed as a candidate. Affirming this hypothesis, we describe herein the specific copurification from human placenta of 46- and 14-kDa proteins by IgG affinity at acid pH. The larger protein, characterized by its amino acid sequence and by immunoblot, is the alpha-chain of human FcRn (hFcRn). The smaller is beta2-microglobulin. Their coisolation by ligand affinity suggests that they comprise the hFcRn heterodimer. Placenta sections stained immunohistochemically with anti-hFcRn alpha-chain peptide Abs show extensive expression of hFcRn in the syncytiotrophoblast and traces in the endothelium and other unidentified cells of the villus stroma. We find alpha-chain mRNA by Northern analysis in human placenta and in human trophoblast-like cell lines (JEG-3, ED27) but not in a human myelocytic cell line (HL60). We suggest that the placental hFcRn heterodimer may transport IgG to the fetus by a mechanism in which maternal IgG is pinocytosed nonspecifically and then carried to fetal tissues by a pH gradient from acidic endosomes to the pH-neutral basolateral surface of the syncytiotrophoblast. Furthermore, the known characteristics of FcRn suggest a wider function, that it is the receptor postulated by Brambell in the 1960s to regulate tissue and serum IgG concentrations by controlling IgG transport and catabolism.
Authors:
J L Leach; D D Sedmak; J M Osborne; B Rahill; M D Lairmore; C L Anderson
Related Documents :
10401997 - The action of flufenamic acid and other nonsteroidal anti-inflammatories on sulfate tra...
10525077 - Molecular cloning and functional characterization of a polyspecific organic anion trans...
9464187 - Na(+)-dependent l-arginine transport in the pigmented rabbit conjunctiva.
17126857 - Effect of thiazolidinediones on bile acid transport in rat liver.
1242107 - Bilayers of dipalmitoyl-3-sn-phosphatidylcholine. conformational differences between th...
20931997 - Enantioselective synthesis of α-fluoro-β(3)-amino esters: synthesis of enantiopure, ort...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  157     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-12-17     Completed Date:  1996-12-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3317-22     Citation Subset:  AIM; IM    
Affiliation:
The Ohio State Biochemistry Program, The Ohio State University, Columbus 43210, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Biological Transport, Active
Female
Histocompatibility Antigens Class I
Humans
Hydrogen-Ion Concentration
Immunity, Maternally-Acquired
Immunoblotting
Immunoglobulin G / metabolism
Immunohistochemistry
Maternal-Fetal Exchange / immunology
Molecular Sequence Data
Molecular Weight
Placenta / immunology*
Pregnancy
RNA, Messenger / genetics
Rats
Receptors, Fc / genetics,  isolation & purification*,  metabolism
Receptors, IgG / genetics,  isolation & purification*,  metabolism
Trophoblasts / immunology
beta 2-Microglobulin / isolation & purification,  metabolism
Grant Support
ID/Acronym/Agency:
R01-AI32744/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Fc receptor, neonatal; 0/Histocompatibility Antigens Class I; 0/Immunoglobulin G; 0/RNA, Messenger; 0/Receptors, Fc; 0/Receptors, IgG; 0/beta 2-Microglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Ultraviolet B radiation sensitizes a murine epidermal dendritic cell line (XS52) to undergo apoptosi...
Next Document:  IL-10 inhibits lipopolysaccharide-induced murine B cell proliferation and cross-linking of surface a...