Document Detail


Isolation from erythrocytes of a green hemoprotein with ferroactivator activity.
MedLine Citation:
PMID:  6783648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A protein that exhibits ferroactivator activity with purified rat liver P-enolpyruvate carboxykinase was purified to apparent homogeneity from bovine erythrocytes. The homogeneous protein has a molecular weight of 82,000 by high speed sedimentation equilibrium and 90,000 by gel exclusion chromatography. A subunit molecular weight of 22,500 was obtained by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Its isoelectric focusing indicated that the ferroactivator protein has an isoelectric point at pH 5.7. The reduced pyridine hemochrome of the denatured protein shows absorption maxima at 419, 527, and 557 nm which is characteristic of protoheme IX. The native protein is green in color with absorption maxima at 407, 507, and 543 nm. The amino acid composition and immunological studies show that bovine erythrocyte protein is structurally similar to the P-enolpyruvate carboxykinase ferroactivator protein isolated from rat liver. Its function in the erythrocyte is unknown.
Authors:
P P Chee; H A Lardy
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  256     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-06-23     Completed Date:  1981-06-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3865-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Cattle
Enzyme Activation
Erythrocytes / metabolism*
Hemeproteins / blood*,  isolation & purification
Immunodiffusion
Liver / enzymology
Macromolecular Substances
Molecular Weight
Phosphoenolpyruvate Carboxykinase (GTP) / metabolism*
Rats
Spectrophotometry
Grant Support
ID/Acronym/Agency:
20678//PHS HHS; AM10,334/AM/NIADDK NIH HHS; DA 05047/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Hemeproteins; 0/Macromolecular Substances; EC 4.1.1.32/Phosphoenolpyruvate Carboxykinase (GTP)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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